Yuanliang Zhai |
|
Assistant
Professor
DNA/RNA
Biology
School of
Biological Sciences
The
University of Hong Kong
Hong Kong,
China
Phone:852-22990653; Email: zhai@hku.hk
|
Education:
|
B.S. |
Sun Yat-Sen University |
2001 |
Microbiology |
|
MPhil |
Sun Yat-Sen University |
2004 |
Biochemistry |
|
Ph.D. |
HKUST |
2010 |
Biochemistry
|
Academic
Positions:
|
Assistant Professor |
06/2018-present |
School of Biological Sciences, HKU |
|
Junior Fellow |
05/2015-05/2018 |
Institute for Advanced Study,
HKUST |
|
Research Assistant Professor |
03/2013-05/2018 |
Super-Resolution
Imaging Center, Division of
Life Science, HKUST |
|
Post-doctoral Fellow |
03/2011-02/2013 |
Division of Life Science, HKUST |
Research Interests:
* Regulation of DNA replication in eukaryotes
*
Cryo-electron microscopy in structure determination
* Advanced optical super-resolution fluorescence
microscopies
Publications (#co-corresponding authors)
1.
Li, N.*, Lam, H.*, Zhai, Y.
*#, Cheng,
J.*, Cheng, E., Zhao, Y., Gao, N.#, and Tye, B.# Structure
of Origin Recognition Complex Bound to ARS DNA. Nature, In press
*
Co-first # co-corresponding authors
2. Zhai, Y.#, Li, N., Jiang, H., Huang, X., Gao, N. #, and Tye,
B. # (2017) Unique roles played by each of the non-identical MCM subunits
in DNA Replication Licensing. Molecular Cell 67, 168-179
3. Zhai, Y.,
Cheng, E., Wu, H., Li, N., Yung, P., Gao, N., and Tye, B. (2017) Open-ringed
Structure of the Cdt1-MCM2-7 Complex as a Precursor of the MCM Double Hexamer. Nature
Structural & Molecular Biology 24, 300-308
4. Zhai, Y.,
and Tye, B. Structure of the MCM2-7 Double Hexamer and Its Implications for the
Mechanistic Functions of the Mcm2-7 Complex. Chapter 10 in "DNA replication:
From Old Principles to New Discovery" Adv Exp Med Biol. 1042:189-205
5. Li,
N.*, Zhai,
Y.*#,
Zhang, Y., Li, W., Yang, M., Lei, J., Tye, B.#, and Gao, N.#
(2015) Structure of the Eukaryotic Minichromosome Maintenance Complex at 3.8 Å. Nature
524, 186-191
*
Co-first # co-corresponding authors
(highlighted in Nature News & Views)
6. Zhao,
T., Wang, Y., Zhai,
Y., Qu, X., Ai, F., Du, S., and
Loy, M. (2015) A user-friendly two-color
super-resolution localization microscope. Optics Express 23, 1879-187
7. Huo, L., Wu, R., Yu, Z., Zhai , Y., Yang, X., Chan, T., Yeung,
J., Kan, J., and Liang, C. (2012) The Rix1 (Ipi1p-2p-3p) Complex Is a Critical
Determinant of DNA Replication Licensing
Independent of Their Roles in Ribosome Biogenesis. Cell Cycle 11, 1325-1339.
8. Zhai, Y., Yung, P., and Liang, C. (2011) Cell cycle control of DNA replication by phosphorylation and dephosphorylation of replication-initiation proteins in budding
yeast. Chapter 6 in "Fundamental Aspects of DNA Replication", InTech Publisher; Ed. J.Kusić-Tisma;
pp87-106.
9. Zhai, Y., Yung,
P., Huo, L., and Liang, C. (2010) Cdc14p
resets the competency of replication licensing by dephosphorylating multiple initiation proteins during mitotic exit in budding yeast. Journal of Cell Science 15;123 :3933-43.
10. Ma, L.*, Zhai, Y.*, Feng, D., Chan,
T., Lu, J., Fu, X., Wang, J., Chen, Y.,
Li, J., Xu, K., and
Liang, C. (2010) Identification of Novel Factors Involved in or Regulating Initiation of DNA Replication by a Phenotypic Screen in Saccharomyces cerevisiae. Cell Cycle 9(21):4399-410.* Co-first authors.
11. Cheng, X., Xu, Z., Wang,
J., Zhai, Y., Lu, Y., and Liang,
C. (2010) ATP-dependent Pre-replicative Complex Assembly Is Facilitated by Adk1p in Budding Yeast.
J. Biol. Chem. 285,
29974-29980.
Research Grants Awarded
Year |
No.
|
|
2017 |
GRF16104617
(PI)
|
Analysis of the in vivo assembled CMG helicase by cryo-EM |
2017 |
N_HKUST614/17
(Co-PI)
|
Cryo-EM Study
of Origin Recognition Complexes Bound with Origin DNA
|
2016 |
GRF16143016 (PI) | Elucidating the structural basis for pre-RC assembly by cryo-EM |
2015 |
GRF16104115 (PI) | Determinant
for DNA binding specificity of the Origin
|
2015 |
IGN15SC02 (PI) | Elucidating
the mechanisms underlying the regulation of
|
2014 |
HKUST12/CRF/13G
(Co-PI)
|
Super-resolution
imaging: revealing the molecular organization of
|
2013 |
GRF664013
(PI)
|
The Role of Mcm10 in Remodeling the Mcm2-7 Ring Structure |
Invited talks and Speaker at International Conferences
| 2018 Mar | Talk at Hong Kong University of Science and Technology, Hong Kong
Talk at Hong Kong University, Hong Kong |
| 2018 Feb | Talk at Indiana University, Bloomington, Indiana, USA Title: Structure informs function: What can we learn about DNA replication mechanisms from the structures of its machines? |
| 2017 Sep | Talk at Cold Spring Harbor meeting on Eukaryotic DNA replication and genome
maintenance,
|
| 2017 Jun | Talk
at Sussex-HKUST Workshop on Genome Damage and Instability, Sussex, UK
Title: Open-ringed Structure of the Cdt1-MCM2-7 Complex as a Precursor of the MCM Double Hexamer |
| 2016 Dec | Talk
at the Departmental Seminar of the Division of Life Science, HKUST, Hong Kong Title: Structure informs function: What can we learn about DNA replication mechanisms from the structures of its machines? |
| 2015 Dec | Talk
at HKUST-Sussex Workshop on Genome Damage and Instability, Hong Kong
Title: The Regulation of DNA Replication Initiation |
| 2014 Jul | Talk
at Gordon Research Conference-genome instability, Hong Kong Title: The Role of Mcm10 in Resolving Replication Forks Stalled at Unfired Replication Origins |
Teaching
| 2018
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BIPH4010
Principles of Quantitative Biology Instrumentation
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| 2017
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Guest
lecture in LIFS 2010 Modern Approaches to Biochemical and Cell Biological
Research
|
| 2017
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Guest
lecture in LIFS 5710 Cellular Regulation
|
| 2017
|
BIPH4010 Principles of
Quantitative Biology Instrumentation
|
| 2006-2007
|
Teaching
Assistant Coordinator, Dept. of Biochemistry, HKUST
|
| 2004-2006
|
Teaching
Assistant, Dept. of Biochemistry, HKUST
|