2010 Journal Publications

  1. Li MWM, Mruk DD, Lee WM, Cheng CY (2010)  Connexin 43 is critical to maintain the homeostasis of blood-testis barrier via its effects on tight junction reassembly.  Proc. Natl. Acad. Sci. U.S.A 107:17998-18003.
  2. Wong EWP, Mruk DD, Lee WM, Cheng CY (2010) Regulation of blood-testis barrier dynamics by TGF-ß3 is a Cdc42-dependent protein trafficking event.  Proc. Natl. Acad. Sci. U.S.A.   107:11399-11404.
  3. Lie PPY, Chan AYN, Mruk DD, Lee WM, Cheng CY (2010) Restricted Arp3 expression in the testis prevents blood-testis barrier disruption during junction restructuring at spermatogenesis.  Proc. Natl. Acad. Sci. U.S.A.  107:11411-11416.
  4. Su L, Mruk DD, Lee WM, Cheng CY. (2010) Differential effects of testosterone and TGF-beta3 on endocytic vesicle-mediated protein trafficking events at the blood-testis barrier. Exp Cell Res. 316:2945-2960.
  5. Lie PPY, Mruk DD, Lee WM, Cheng CY (2010) Cytoskeletal dynamics and spermatogenesis.  Philos Trans R Soc B Biol Sci  365:1581-1592.

2009 Journal Publications

  1. Li MWM, Mruk DD, Lee WM, Cheng  CY (2009) Connexin 43 and plakophilin-2 as a signaling complex that regulates blood-testis barrier dynamics. Proc. Natl. Acad. Sci. U.S.A. 106:10213-10218.
  2. Lie PPY, Mruk DD, Lee WM and Cheng CY (2009) Epidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium. FASEB J 23:2555-2567.
  3. Lui WY and Lee WM (2009)  Molecular mechanisms by which hormones and cytokines regulate cell junction dynamics in the testis. J. Mol. Endocrinol. 43:43-51. 
  4. Wong EWP, Sun IS, Li MWM, Lee WM, Cheng CY (2009) 14-3-3 regulates cell adhesion in the seminiferous epithelium.  Endocrinology 150:4713-4723.
  5. Sun IS, Wong EWP, Li MWM, Lee WM, Cheng CY (2009) 14-3-3 and its binding partners are regulators of protein-protein interactions in spermatogenesis.  J. Endocrinol.  202:327-336.
  6. Li MWM, Mruk DD, Lee WM, Cheng  CY (2009) Disruption of the blood-testis barrier integrity by bisphenol A in vitro: Is this a suitable model for studying blood-testis barrier dynamics?  International Journal of Biochemistry & Cell Biology.  41:2302-2314.
  7. Li MWM, Mruk DD, Lee WM, Cheng  CY (2009) Cytokines and the junction restructuring events during spermatogenesis in the testis: an emerging new concept of regulation.  Cytokine and Growth Factor Reviews 20:329-338.

2008 Journal Publications

  1. Sze KL, Lui WY and Lee WM (2008) Post-transcriptional regulation of CLMP mRNA is controlled by tritetraprolin in response to TNFα via c-Jun N-terminal kinase signaling Biochemical Journal  410:575-583.
  2. Lui WY and Lee WM (2008) Ubiquitination system in male reproduction and its relevance to contraception.  Immun., Endoc. & Metab. Agents in Med. Chem. 8:14-19.
  3. Lui WY and Lee WM (2008)  Mechanisms of reorganization of cell-cell junctions in the testis.  Frontiers in Biosciences 13:6775-6786.  
  4. Yan HHN, Wong EWP, Mruk DD, Lee WM and Cheng CY (2008) An autocrine axis in the testis that coordinates spermiation and blood-testis barrier restructuring during spermatogenesis.  Proc. Natl. Acad. Sci. U.S.A. 105:8050-8055.
  5. Wong EWP, Mruk DD, Lee WM and Cheng CY (2008) Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis.   Proc. Natl. Acad. Sci. U.S.A. 105:9657-9662.
  6. Sze KL, Lee WM and Lui WY (2008) Expression of CLMP, a novel tight junction protein, is mediated via the interaction of GATA with the Kruppel family proteins, KLF4 and Sp1, in mouse TM4 Sertoli cells.  Journal of Cellular Physiology 214:334-344.
  7. Yan HHN, Mruk DD, Lee WM and Cheng CY (2008) Blood-testis barrier dynamics are regulated by testosterone and cytokines via their differential effects on the kinetics of protein endocytosis and recycling in Sertoli cells.  FASEB Journal 22:1945-1959.

 

2007 Journal Publications

 

  1. Lui WY, Wong EYP, Guan YF and Lee WM (2007) Dual transcriptional control of claudin-11 via an overlapping GATA/NF-Y motif: positive regulation through the Interaction of GATA, NF-YA, and CREB and negative Regulation through the interaction of Smad, HDAC1, and mSin3A.  Journal of Cellular Physiology 211:638-648.
  2. Yan HHN, Mruk DD, Lee WM and Cheng CY (2007) Ectoplasmic specialization is a unique hybrid anchoring and tight junction in the testis -- a friend or a foe of spermatogenesis?  BioEssays 29:36-48.
  3. Xia WL, Mruk DD, Lee WM and Cheng CY (2007) Unraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the Adjudin-induced germ cell depletion model.  Journal of Endocrinology 192:563-583.
  4. Wang CQF, Mruk DD, Lee WM and Cheng CY (2007)  Coxsackie and adenovirus receptor (CAR) is a product of Sertoli and germ cells in adult rat testes which is localized at the SertoliSertoli and Sertoli-germ cell interface.  Experimental Cell Research 313:1373-1392.
  5. Wong CH, Mruk DD, Lee WM, Cheng CY (2007) Targeted and reversible disruption of the blood-testis barrier induced by an FSH mutant-occludin peptide conjugate.  FASEB Journal 21:438-448.

 

2006 Journal Publications

 

  1. Lui WY, Sze KL and Lee WM (2006)  Nectin-2 expression in testicular cells is controlled via the functional cooperation between transcription factors of the Sp1, CREB, and AP-1 families.  Journal of Cellular Physiology 207:144-157.
  2. Lui WY and Lee WM (2006)  Transcriptional and post-translational regulations of cell-junction proteins in the testis.  Molecular & Cellular Endocrinology 250:25-35.
  3. Xia WL, Mruk DD, Lee WM and Cheng CY (2006) Differential interactions between TGF-b3/TbR1 and adaptors TAB1 and CD2AP selectively disrupt blood-testis barrier and Sertoli-germ cell adhesion. Journal of Biological Chemistry 281:16799-16813.
  4. Li MWM, Xia WL, Mruk DD, Wang Q, Yan HHN, Siu MKY, Lui WY, Lee WM and Cheng CY (2006) TNFa can reversibly disrupt the blood-testis barrier (BTB) and impair Sertoli-germ cell adhesion – a novel mechanism to regulate junction dynamics during spermatogenesis.  Journal of Endocrinology 190:313-329.
  5. Lie PPY, Xia WL, Wang Q, Mruk DD, Yan HHN, Wong CH, Lee WM and Cheng CY (2006) Dynamin II interacts with the cadherin- and occludin-based protein complexes at the blood-testis barrier in adult rat testes. Journal of Endocrinology 191:571-586.

 

2005 Journal Publications

  1. Lui WY and Lee WM (2005) cAMP perturbs inter-Sertoli tight junction permeability barrier in vitro via its effect on proteasome-sensitive ubiquitination of occludin. Journal of Cellular Physiology 203: 564-572.
  2. Ng KM, So MT and Lee WM (2005) Expression of rabbit sex hormone-binding globulin during pregnancy and prenatal development and identification of a novel isoform. Endocrinology 146:1965-1972.
  3. Wong CH, Xia WL, Lee NPY, Mruk DD, Lee WM and Cheng CY (2005) Regulation of ectoplasmic specialization dynamics in the seminiferous epithelium by focal adhesion-associated proteins in testosterone-suppressed rat testes. Endocrinology 146:1192-1204.
  4. Zhang JY, Wong CH, Xia WL, Mruk DD, Lee NPY, Lee WM and Cheng CY (2005) Regulation of Sertoli-germ cell adherens junction dynamics via changes in protein-protein interactions of the N-cadherin-b -catenin protein complex which are possibly mediated by c-Src and MTMR2: an in vivo study using an androgen suppression model. Endocrinology 146:1268-1284.
  5. Xia WL, Wong CH, Lee NPY, Lee WM and Cheng CY (2005) Disruption of Sertoli-germ cell adhesion function in the seminiferous epithelium of the rat testis can be limited to adherens junctions without affecting the blood-testis barrier integrity: an in vivo study using an androgen suppression model. Journal of Cellular Physiology 205:141-157.
  6. Xia WL, Mruk DD, Lee WM and Cheng CY (2005) Cytokines and junction restructuring during spermatogenesis – a lesson to learn from the testis. Cytokine & Growth Factor Reviews 16:469-493.
  7. Siu MKY, Wong CH, Lee WM, Cheng CY (2005) Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases. Journal of Biological Chemistry 280:25029-25047.
  8. Lui WY, Cheng YH, Cheng CH, Mo MY, Mruk DD, Lee WM, Cheng CY (2005) α2-Macroglobulin expression in the liver in response to inflammation is mediated by the testis. Journal of Endocrinology 185:497-505.

2004 Journal Publications

  1. Lee YL, Lee KF, Xu JS, He QY, Chiu JF, Lee WM, Luk JM, and Yeung WSB (2004) The embryotrophic activity of oviductal cell derived complement C3b and iC3b--a novel function of complement protein in reproduction. Journal of Biological Chemistry 279:12763-12768.
  2. Xu JS, Lee YL, Lee KF, Kwok KL, Lee WM, Luk JM, Yeung WSB (2004) Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the b 1 subunit of sodium-potassium ATPase in mouse embryos. Human Reproduction 19: 2919-2926. Epub 2004 Sep 30.
  3. Xu JS, Chan STH, Lee WM, Lee KF and Yeung WSB (2004) Differential growth, cell proliferation, and apoptosis of mouse embryo in various culture media and in coculture. Molecular Reproduction and Development 68:72-80.

2003 Journal Publications

  1. Cheng KCK, Cheung CH and Lee WM (2003) Mouse Testin: complementary DNA cloning, genomic organization, and characterization of its proximal promoter region. Biology of Reproduction 68:1376-1386.
  2. Lee NPY, Mruk D, Lee WM and Cheng CY (2003) Is the cadherin/catenin complex a functional unit of cell-cell actin-based adherens junctions in the rat testis? Biology of Reproduction 68:489-508.
  3. Siu MKY, Lee WM and Cheng CY (2003) The interplay of collagen IV, tumor necrosis factor-a , gelatinase B (Matrix Metalloprotease-9), and tissue inhibitor of metalloprotease-1 in the basal lamina regulates Sertoli cell-tight junction dynamics in the rat testis. Endocrinology 144:371-387.
  4. Siu MKY, Mruk D, Lee WM and Cheng CY (2003) Adhering junction dynamics in the testis are regulated by an interplay of b 1-integrin and the focal adhesion complex (FAC)-associated proteins. Endocrinology 144:2141-2163.
  5. Lui WY, Mruk D, Lee WM and Cheng CY (2003) Sertoli cell tight junction dynamics: their regulation and spermatogenesis. Biology of Reproduction 68:1087-1097.
  6. Lui WY, Lee WM and Cheng CY (2003) Transforming growth factor-b 3 regulates the dynamics of Sertoli cell tight junctions via the p38 mitogen-activated protein kinase pathway. Biology of Reproduction 68:1597-1612.
  7. Lui WY, Mruk D, Lee WM and Cheng CY (2003) Adherens junctions (AJ) dynamics in the testis and spermatogenesis. Journal of Andrology 24:1-14.
  8. Lui WY, Lee WM and Cheng CY (2003) TGF-b s: their role in testicular function and Sertoli cell tight junction dynamics. International Journal of Andrology 26:147-160.
  9. Lui WY, Lee WM and Cheng CY (2003) Rho GTPases and spermatogenesis. Biochim Biophys Acta 1593:121-129.
  10. Lui WY, Lee WM and Cheng CY (2003) Sertoli-Germ Cell Adherens Junction Dynamics in the Testis Are Regulated by RhoB GTPase via the ROCK/LIMK Signaling Pathway
    Biology of Reproduction 68:2189-2206.
  11. Yao YQ, Xu JS, Lee WM, Yeung WS and Lee KF (2003) Identification of mRNAs that are up-regulated after fertilization in the murine zygote by suppression subtractive hybridization. Biochem Biophys Res Comm. 304:60-66.
  12. Chen Y-m, Lee NPY, Mruk DD, Lee WM, and Cheng CY (2003) Fer kinase/FerT and adherens junction dynamics in the testis: an in vitro and in vivo study. Biology of Reproduction 69:656-672.
  13. Lee YL, Lee KF, Xu JS, Kwok KL, Luk JM, Lee WM and Yeung WSB (2003) Embryotrophic factor-3 from human oviductal cells affects the messenger ribonucleic acid expression of mouse blastocyst. Biology of Reproduction 68:375-382.

2002 Journal Publications

  1. Wong CCS and Lee WM (2002) Proximal cis-acting elements Sp1, Sp3 and E2F regulate the mouse Mer gene transcription in Sertoli cells. European Journal of Biochemistry 269:3789-3800.

2001 Journal Publications

  1. Lui WY, Lee WM and Cheng CY (2001) Transforming growth factor-beta3 perturbs the inter-Sertoli tight junction permeability barrier in vitro possibly mediated via its effects on occludin, zonula occludens-1, and claudin-11. Endocrinology 142:1865-1877.
  2. Li JCH, Lee WM, Mruk D, and Cheng CY (2001) Regulation of Sertoli cell myotubularin (rMTM) expression by germ cells in vitro. Journal of Andrology 22:266-277.
  3. Wong AST, Lui WY, Hui ITC and Lee WM (2001) Rabbit sex hormone-binding globulin: expression in the liver and testis during postnatal development and structural characterization by truncated proteins. International Journal of Andrology 24:165-174.
  4. Chung NPY, Mruk D, Mo MY, Lee WM and Cheng CY (2001) A 22-amino acid Synthetic peptide corresponding to the second extracellular loop of rat occludin perturbs the blood-testis barrier and disrupts spermatogenesis reversibly in vivo. Biology of Reproduction 65:1340-1351.

2000 Journal Publications

  1. Samy ET, Li JCH, Grima J, Lee WM, Silvestrini B and Cheng CY (2000) Sertoli cell prostaglandin D2 synthetase (PGD-S) is a multifunctional molecule: its expression and regulation. Endocrinology 141:710-721.
  2. Ip YC, Hammond GL and Lee WM (2000) The rabbit sex hormone-binding globulin gene: structural organization and characterization of its 5’ flanking region. Endocrinology 141:1356-1365.
  3. Wong CCS, Chung SSW, Mruk D, Lee WM and Cheng CY (2000) Changes in the expression of junctional and non-junctional complex component genes when inter-Sertoli tight junctions are formed in vitro. Journal of Andrology 21:227-237.
  4. Chan MCW, Mather JP, McCray G and Lee WM (2000) Identification and regulation of tyrosine kinase receptors RSE and MER, and their ligand GAS6, in the testicular somatic cells. Journal of Andrology 21:291-302.
  5. Li JCH, Chung SSW, Samy ET, Mruk D, Grima J, Lee WM, Silvestrini B and Cheng CY (2000) Rat myotubularin (rMTM) is a Sertoli cell product which can be used to monitor cell-cell interactions in the testis. Journal of Cellular Physiology 185: cover page and 366-385.

1999 Journal Publications

  1. Chung SSW, Lee WM and Cheng CY (1999) A study on the formation of specialized inter-Sertoli cell junctions in vitro. Journal of Cellular Physiology 181:258-272.
  2. Wong PYD, Chan HC, Leung PS, Wong YL, Lee WM, Ng V and Dun NJ (1999) Regulation of anion secretion by cyclooxygenase and prostanoids in cultured rat epididymal epithelia. Journal of Physiology-London 514:809-820.
  3. Chung SSW, Mruk D, Lee WM and Cheng CY (1999) Identification and purification of proteins from germ cell-conditioned medium (GCCM). Biochemistry and Molecular Biology International 47:479-491.
  4. Chung SSW, Lee WM and Cheng CY (1999) An in vitro study on the formation of specialized inter-Sertoli cell junctions. In: Recent Advances in Reproductive Medicine. 10th World Congress on Human Reproduction. Eds. Coutinho E and Spinola P., Monduzzi Editore S.p.A., Bologna (Italy) pp. 29-32.
  5. Cheng CY, Mo MY, Mruk D, Lee WM and Silvestrini B (1999) TEMO is a novel testicular gene to study Sertoli-germ cell interactions: Its cDNA cloning and regulation. In: Recent Advances in Reproductive Medicine. 10th World Congress on Human Reproduction. Eds. Coutinho E and Spinola P., Monduzzi Editore S.p.A., Bologna (Italy) pp. 89-92.

1998 Journal Publications

  1. Chung SSW, Mo MY, Silvestrini B, Lee WM and Cheng CY (1998) Rat testicular N-cadherin: its complementary deoxyribonucleic acid cloning and regulation. Endocrinology 139: 1853-1862.
  2. Mruk D, Cheng CH, Cheng YH, Mo MY, Grima J, Silvestrini B, Lee WM and Cheng CY  (1998)  Rat testicular extracellular superoxide dismutase   (SODEX):  its purification, cellular distribution,  and regulation. Biology of Reproduction 59: 298-308.
  3. Chung SSW, Zhu LJ, Mo MY, Silvestrini B, Lee WM  and  Cheng, CY  (1998) Evidence for cross-talk between Sertoli and germ cells using selected cathepsins as markers. Journal of Andrology 19: 686-703.

1997 Journal Publications

  1. Lee WM, Wong AST, Tu AWK, Cheung CH, Li JCH and Hammond GL (1997) Rabbit sex hormone binding globulin: primary structure, tissue expression, and structure/function analyses by expression in Escherichia coli.   Journal of Endocrinology 153: 373-384.
  2. Aravindan GR, Mruk D, Lee WM and Cheng CY  (1997)  Identification, isolation, and characterization of a 41-kilodalton protein from rat germ cell-conditioned medium exhibiting concentration-dependent dual biological activities. Endocrinology 138: 3259-3268.
  3. Mathur PP,  Grima J, Mo MY, Zhu LJ, Aravindan GR,  Calcagno K,  O'Bryan M, Silvestrini B, Chung SSW, Mruk D, Lee WM  and Cheng CY (1997)  Differential expression of multiple cathepsin mRNAs in the rat testis during development, maturation, and tissue restructuring. Biochemistry and Molecular Biology International 42:217-233.
  4. Mruk D, Zhu LJ, Silvestrini B, Lee WM and Cheng CY (1997)   Interactions of proteases and protease inhibitors in Sertoli-germ cell co-cultures preceding the formation of specialized Sertoli-germ cell junctions in vitro. Journal of  Andrology 18: 612-622.