Prof. Steven Feng Chen () I. Food Biotechnology Laboratory II. Selected Recent Publications Ph.D. Studentships

I. Food Biotechnology Laboratory

This Laboratory was first established in 1993 by Prof. Steven Feng Chen when he joined the University of Hong Kong as an Assistant Professor. The Laboratory is mainly supported by University Research Grants and External Research Grants. Major grants awarded include 10 RGC (GRF) grants, 2 ITF grants and 1 EPD grant, totaling more than 12 million HK dollars. Additional funding (more than 3 million HK dollars) from CRCG grants, URC grants, teaching grants and equipment grants has been received. The Laboratory is very well equipped with modern facilities, such as 10 fermentors (Bioengineering, Switzerland) ranging from 2.4-L to 30-L, 3 HPLC systems (Beckman and Waters), 1 GC system (HP), 1 GC-MS system (HP), 1 counter-current chromatographic system, 1 PCR machine, 1 gene pulser (BIO-RAD), 1 gel doc and many others. There are also shared large equipment items in the Central Laboratories of School of Biological Sciences.

The laboratory is led by Prof. Steven Feng Chen. Prof. Chen is a member of the Editorial Board of the following international journals: Process Biochemistry (Elsevier), Biotechnology Letters (Springer), Journal of Applied Phycology (Springer) and Open Food Science Journal (Bentham). Prof. Chen is also a Director of Chinese Institute of Food Science and Technology, Founding Council Member of Asia-Pacific Society for Applied Phycology, Founding President of Hong Kong Proteomics Society, Guest (or Consultant) Professor of several universities in mainland China. Professor Chen is a Recipient of the prestigious Outstanding Young Researcher Award of The University of Hong Kong (2002-2003), Outstanding Research Student Supervisor Award of the University of Hong Kong (2005-2006), Outstanding Researcher in Food Science and Technology Award (2004), CIFST Science and Technology Innovation Award (2008), and Elected Fellow of American Institute for Medical and Biological Engineering (AIMBE). In his capacity as Chairman, Prof. Chen has organized several international conferences including the Fourth Asia-Pacific Conference on Algal Biotechnology.

Main Research Interests:

• High-yield production of astaxanthin, lutein, phycocyanin, long-chain polyunsaturated fatty acids, and bioactive proteins by microalgae and cyanobacteria in heterotrophic and mixotrophic cultures.
• Mechanism of formation of secondary metabolites in microalgae in heterotrophic culture.
• Metabolic engineering of carotenoid biosynthesis and glucose transportation in green microalgae and cyanobacteria.
  Biodiesels from microalgae – process optimization and genetic manipulation.
• Fermentation technology including bioreactor designs, high cell density techniques, mechanisms of substrate inhibition, downstream processing of fermentation products, and modelling of fermentation processes, etc.
• Characterization of anti-cancer activities of bioactive compounds from Chinese herbs or algae by proteomics.
• Separation and purification of lipids and secondary metabolites from microalgae and Chinese medicinal plants by High-Speed Counter Current Chromatography (HSCCC).
• Inhibitory effects and mechanisms of dietary polyphenolic compounds on the formation of toxic substances from Mailard reaction.
• Natural products as reactive carbonyl scavengers to prevent formation of advanced glycation endproducts.
• Identification and determination of various drugs and food and fermentation compounds by molecular imprinting, GC, HPLC, LC-MS and related techniques.
• Chinese medicine for the treatment of SARS, diabetes, heart diseases, and cancers as well as their complications.

Members in The Lab:

There are currently 10 Ph.D. students, two postdoctoral associates, one senior research assistant, one lab manager (Dr. Keith K.W. Fan), and usually two or three visitors or trainees.

Recent Degree Completion:

Ph.D.

• Prof. Yi-Ming Zhang, Ph.D. (1996) (co-supervision with Prof. S.Y. Guo of SCUT)
• Dr. Xian-Di Gong, Ph.D. (1997)
• Prof. Xian-Ming Shi, Ph.D. (1998)
• Dr. Yue Jiang, Ph.D. (1998) (co-supervision with Prof. S.Z. Liang of SCUT)
• Dr. Rema Vazhappilly, Ph.D. (1998)
• Prof. Gui-Xing Ren, Ph.D. (1998)
• Dr. Jian-Ping Yuan, Ph.D. (1999)
• Prof. Hui Chen, Ph.D. (2000)
• Dr. Zhi-You Wen, Ph.D. (2001)
• Dr. Raymond Yin-Nin Ma, Ph.D. (2001)
• Dr. Sheng-Bing Wang, Ph.D. (2003)
• Dr. Siva S.K. Durairajan, Ph.D. (2004)(co-supervision with Dr. K.D. Hyde)
• Dr. Xue-Jun Liu, Ph.D. (2004)
• Dr. Mabel P.F. Ip (2005)
• Dr. Yan-Tao Li (2006)
• Dr. Guan-Qun Chen (2007)
• Dr. Sze-Yuen Ho (2008)

M. Phil.

• Ms. Oi-Ha Lau, M.Phil. (1998)
• Ms. Ping-Ping Lam, M. Phil. (1999)
• Miss Suk-Man Cheung, M. Phil. (1999)
• Miss Chui-Yee Yap, M. Phil. (2001)
• Mr. Ka-Ho Wong, M.Phil. (2006)
• Miss Jeanie K.Y. Tsui, M.Phil. (2006)
• Mr. Dennis Chi-Chun Wong (2007)

II. Selected Publications in International Journals (since 1996)

196. Fan, K.W., Jiang, Y., Ho, L.T. & Chen, F.* (2009). Differentiation in fatty acid profiles of pigmented and non-pigmented Aurantiochytrium isolated from Hong Kong mangroves. Journal of Agricultural and Food Chemistry (in press).

195. Peng, X.F., Ma, J.Y., Cheng, K.W., Jiang, Y., Chen, F. & Wang, M.F. (2009). The effects of grape seed extract fortification on the antioxidant activity and quality attributes of bread. Food Chemistry (in press).

194. Huang, G.H., Chen, G. & Chen, F. (2009). Application of Nile red in rapid screening of enhanced lipid production in Chlorella. Biomass & Bioenergy (in press).

193. Li, Y.T., Sommerfeld, Chen, F.* & Hu, Q. (2009). Effect of photon flux densities on regulation of carotenogenesis and cell viability of Haematococcus pluvialis (Chlorophyceae). Journal of Applied Phycology (in press).

192. Pahl, S.L., Lewis M.D., Chen, F. & King, K.D. (2009). Growth dynamics and the proximate biochemical composition and fatty acid profile of the heterotrophically grown diatom Cyclotella cryptica. Journal of Applied Phycology (in press).

191. Xu, Y., Chiu, J.F., He, Q.Y. & Chen, F.* (2009). Tubeimoside-1 exerts cytotoxicity in HeLa cells through mitochondrial dysfunction and endoplasmic reticulum stress pathways. Journal of Proteome Research, 8, 1585-1593.

190. Zeng, X.H., Cheng, K.W., Jiang, Y., Lin, Z.X., Shi, J.J., Ou, S.Y., Chen, F. & Wang, M.F. (2009). Inhibitory of acrylamide formation by vitamins in model reactions and fried potato strips. Food Chemistry, 116, 34-39.

189. Chen, T., Wei, D., Chen, G., Wang, Y. & Chen, F.* (2009). Employment of organic acids to enhance astaxanthin formation in heterotrophic Chlorella zofingiensis. Journal of Food Processing and Preservation, 33, 271-284 (invited paper).

188. Li, H.B., & Chen, F. (2009). Separation and purification of epimedin A, epimedin B, epimedin C and icariin from the medicinal herb Epimedium brevicornum Maxim by dual-mode high-speed counter-current chromatography. Journal of Chromatographic Science (in press).

187. Xu, X.R., Li, S.X., Li, X.Y., Gu, J.D., Chen, F., Li, X.Z. & Li, H.B. (2009). Degradation of n-butyl benzyl phthalate using TiO2/UV. Journal of Hazardous Materials, 164, 527-532.

186. Zhu, Q., Liang, C.P., Cheng, K.W., Peng, X.F., Lo, C.Y., Shahidi, F., Chen, F., Ho, C.T. & Wang, M.F. (2009). Trapping effects of green and black tea extracts on peroxidation-derived carbonyl substances of seal blubber oil. Journal of Agricultural and Food Chemistry, 57, 1065-1069.

185. Wei, D., Chen, F., Chen, G., Zhang, X.W., Liu, L.J. & Zhang, H. (2008). Enhanced production of lutein in heterotrophic Chlorella protothecoides by oxidative stress. Science in China Series C: Life Sciences, 51, 1088-1093.

184. Peng, J., Xiang, W.Z., Tang, Q.M., Sun, N. Chen, F. & Yuan, J.P. (2008). Comparative analysis of astaxanthin and its esters in the mutant E1 of Haematococcus pluvialis and other green algae by HLPC with a C30 column.  Science in China Series C: Life Sciences, 51, 1108-1115.

183. Li, Y.T., Huang, J.C., Sandmann, G. & Chen, F.* (2009). High-light and sodium chloride stress differently regulate the biosynthesis of astaxanthin in Chlorella zofingiensis (Chlorophyceae). Journal of Phycology, 45, 635-641.

182. Yao, Y., Chen, F., Wang, M.F., Wang, J.S. & Ren, G.X. (2008). Antidiabetic activity of mung bean extracts in diabetic KK-Ay mice. Journal of Agricultural and Food Chemistry, 56, 8869-8873.

181. Yao, Y., Shan, F., Bian, J.S., Chen, F., Wang, M.F. & Ren, G.X. (2008). D-chiro-inositol enriched tartary buckwheat bran extract lowers the blood glucose level in KK-Ay mice. Journal of Agricultural and Food Chemistry, 56, 10027-10031.

180. Cheng, KW, Wong, CC, Chao, J, Lo, C, Chen, F, Chu, IK, Che, C, Ho, CT, Wang, M. (2008). Inhibition of mutagenic PhIP formation by EGCG via scavenging of phenylacetaldehyde. Molecular Nutrition and Food Research (in press)

179. Cheng, KW, Wong, CC, Wang, M, He, QY, Chen, F.* (2008). Identification and characterization of molecular targets of natural products by mass spectrometer. Mass Spectrometry Reviews, (invited review, in press).

178. Sun, N., Wang, Y., Huang, J.C. & Chen, F.* (2008). Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta). Process Biochemistry, 43, 1288-1292.

177. Cheng, K.W., Wong, C.C., Cho, C.K., Chu, I.K., Sze, K.H., Lo, C., Chen, F. & Wang, M.F. (2008). Trapping of phenylacetaldehyde as a key mechanism responsible for naringenin’s inhibitory activity in mutagenic 2-amino-1-methyl-6-phenylimidazo [4,5-b]phyridine (PhIP) formation. Chemical Research in Toxicology, 21, 2026-2034.

176. Li, Y.T., Huang, J.C., Sandmann, G. & Chen, F.* (2008). Glucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae). Planta, 228, 735-743.

175. Ho, S.Y. & Chen, F.* (2008). Lipid characterization of Mortierella alpina grown at different NaCl concentrations. Journal of Agricultural and Food Chemistry, 56, 7903-7909.

174. Ma, J.Y., Peng, X.F., Cheng, K.W., Chen, B., Chen, F. & Wang, M.F. (2008). Use of capillary electrophoresis to evaluate protective effects of methylglyoxal scavengers on the activity of creatine kinase.  Journal of Separation Science, 31, 2846-2851.

173. Peng, X.F., Ma, J.Y., Cheng, K.W., Chen, B., Chen, F. & Wang, M.F. (2009). High performance liquid chromatographic determination of creatine kinase activity influenced by methylglyoxal. Biomedical Chromatography, 23, 170-174.

172. Hu, Z.Y., Li, Y.T., Sommerfeld, M, Chen, F. & Hu, Q. (2008). Enhanced protection against oxidative stress in an astaxanthin overproduction Haematococcus mutant (Chlorophyceae). European Journal of Phycology, 43, 365-376.

171. Ho, S.Y. & Chen, F.* (2008). Genetic characterization of Mortierella alpina by sequencing the 18S-28S ribosomal gene internal transcribed spacer region. Letters in Applied Microbiology, 47, 250-255.

170. Chen, G.Q., Jiang, Y. & Chen, F.* (2008). Salt-induced alterations in lipid composition of diatom Nitzshia laevis (Bacillariophyceae). Journal of Phycology, 44, 1309-1314.

169. Li, Y.Q., Zhang, X.W., Chen, G., Wei, D. & Chen, F. (2008). Algal lectins for prevention of HIV transmission. Current Medicinal Chemistry, 15, 1096-1104.

168. Wong, C.C., Wang, Y., Cheng, K.W., Chiu, J.F., He, Q.Y. & Chen, F.* (2008). Comparative proteomic analysis of indioside D-triggered cell death in HeLa cells. Journal of Proteome Research, 7, 2050-2058.

167. Tsui, J.K.Y., Guenther, A., Yip, W.K. & Chen, F.* (2008). A biogenic volatile organic compound emission inventory for Hong Kong.  Atmospheric Environment (in press).

166. Peng, X.F., Cheng, K.W., Ma, J.Y., Chen, B., Ho, C.T., Chen, F. & Wang, M.F. (2008). Cinnamon bark proanthocyanidins as reactive carbonyl scavengers to prevent the formation of advanced glycation endproducts. Journal of Agricultural and Food Chemistry 56, 1907-1911.

165. Li, Y.T., Chen, F., Sommerfeld, M. Hu, Q. (2008). Consumption of oxygen by astaxanthin biosynthesis: a protective mechanism against oxidative stress in Haematococcus pluvialis (Chlorophyceae). Journal of Plant Physiology (in press).

164. Huang, J.C., Liu, J., Li, Y.T. & Chen, F.* (2008). Isolation and characterization of the phytoene desaturase gene as a potential selective marker for genetic engineering of the astaxanthin-producing green alga Chlorella zofingiensis (Chlorophyta). Journal of Phycology, 44, 684-690.

163. Chen, G.Q., Jiang, Y. & Chen, F.* (2008). Variation of lipid class composition in Nitzschia laevis as a response to growth temperature change. Food Chemistry, 109, 88-94.

162. Cheng, K.W., Wu, Q., Zheng, Z.P., Peng, X.F., Simon, J.E., Chen, F. & Wang, M.F. (2007). Inhibitory effect of fruit extracts on the formation of heterocyclic amines. Journal of Agricultural and Food Chemistry, 55, 10359-10365.

161. Zhang, X.W., Yap, Y., Wei, D., Chen, G. & Chen, F. (2008). Novel omics technologies in nutrition research. Biotechnology Advances, 26, 169-176.

160. Peng, X.F., Zheng, Z.P., Cheng, K.W., Shan, F., Ren, G.X., Chen, F., Wang, M.F. (2008). Inhibitory effect of mung bean extract and its constituents vitexin and isovitexin on the formation of advanced glycation endproducts.  Food Chemistry, 106, 475-481.

159. Fan, K.W., Fok, L., Lee, J.H.W. & Chen, F.* (2007). Epilithic diatom as a biological indicator for the assessment of water quality in the East River. Water Science & Technology: Water Supply, 7(2), 147-155.

158. Wong, C.C., Cheng, K.W., He, Q.Y. & Chen, F.* (2008). Unraveling the molecular targets of natural products: Insights from genomic and proteomic analyses. Proteomics – Clinical Application, 2, 338-354..

157. Cheng, K.W., Chen, F. & Wang, M.F. (2007). Inhibitory activities of dietary phenolic compounds on heterocyclic amine formation in both chemical model system and beef patties. Molecular Nutrition & Food Research, 51, 969-976.

156. Zhang, Y.F., Wei, D., Guo, S.Y., Zhang, X.W., Wang, M.F. & Chen, F. (2007). Chemical components and antioxidant activity of the volatile oil from Cassia tora L. seed prepared by supercritical fluid extraction. Journal of Food Lipids, 14, 411-423.

155. Ho, S.Y., Jiang, Y. & Chen, F.* (2007). Polyunsaturated fatty acids (PUFAs) content of fungus Mortierella alpina isolated from soil. Journal of Agricultural and Food Chemistry, 55, 3960-3966.

154. Li, H.B., Wong, C.C., Cheng, K.W. & Chen, F. (2008). Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT – Food Science and Technology, 41, 385-390.

153. Chen, G.Q., Jiang, Y. & Chen, F.* (2007). Fatty acid and lipid class composition of the eicosapentaenoic acid-producing microalga, Nitzschia laevis. Food Chemistry, 104, 1580-1585.

152. Li, H.B., Jiang, Y., Wong, C.C., Cheng, K.W. & Chen, F.* (2007). Evaluation of two extraction methods for extraction of antioxidants from medicinal plants. Analytical and Bioanalytical Chemistry, 388, 483-488.

151. Fan, K.W., Jiang, Y., Faan, Y.W. & Chen, F.* (2007). Lipid characterization of mangrove thraustochytrid – Schizochytrium mangrovei. Journal of Agricultural and Food Chemistry, 55, 2906-2910.

150. Zhang,X.W., :Li, L., Wei, D., Yap, Y. & Chen, F.* (2007). Moving cancer diagnostics from bench to bedside. Trends in Biotechnology, 25, 166-173.

149. Zhang,X.W., Wei, D., Yap, Y., Li, L., Guo, S.Y. & Chen, F.*(2007). Mass spectrometry-based “omics” technologies in cancer diagnostics. Mass Spectrometry Reviews, 26, 403-431.

148. Li, H.B., Cheng, K.W., Wong, C.C., Fan, K.W. & Chen, F.*, Jiang, Y. (2007). Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae.  Food Chemistry, 102, 771-776.

147. Cheng, K.W., Chen, F. & Wang, M.F. (2006). Heterocyclic amines: chemistry and health. Molecular Nutrition & Food Research. 50, 1150-1170.

146. Hu, J.M., Wei, D., Guo, S.Y. & Chen, F. (2006). Effects of environmental factors and denaturants on the specific absorption spectrum and fluorescence characters of B-phycoerythrin. Spectroscopy and Spectral Analysis, 26, 2258-2263.

145. Shi, X.M., Wu, Z.Y. & Chen, F. (2006). Kinetic modelling of lutein production by heterotrophic Chlorella at various pH and temperatures. Molecular Nutrition & Food Research, 50, 763-768.

144. Chen, G.Q. & Chen, F.* (2006). Growing phototrophic cells without light. Biotechnology Letters, 28, 607-616. [invite review].

143. Huang, J.C., Wang, Y., Sandmann, G., Chen, F.* (2006). Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta). Applied Microbiology and Biotechnology, 71, 473-479.

142. Li, H.B., Fan, K.W. & Chen, F.* (2006). Isolation and purification of canthaxanthin from the microalga Chlorella zofingiensis by high-speed counter-current chromatography. Journal of Separation Science, 29(5), 699-703.

141. Huang, J.C., Chen, F. * & Sandmann G.  (2006). Stress-related differential expression of multiple b-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis.  Journal of Biotechnology, 122, 176-185.

140. Huang, J.C. & Chen, F.* (2006). Simultaneous amplification of 5’ and 3’ cDNA ends based on template-switching effect and inverse PCR.  Biotechniques, 40, 187-189.

139. Wong, C.C., Li, H.B., Cheng, K.W. & Chen, F.* (2006). A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay.  Food Chemistry, 97, 705-711.

138. Wang, S.B., Chen, F.*, Sommerfeld, M. & Hu, Q. (2005). Isolation and characterization of cell wall-deficient Haematococcus pluvialis mutants.  Proteomics, 5, 4839-4851. [featured in the cover of the journal].

137. Zhang, X.W., Yap, Y.L., Wei, D., Chen, F. & Danchin, A. (2005). Molecular diagnosis of human cancer type by gene expression profiles and independent component analysis. European Journal of Human Genetics, 13, 1303-1311.

136. He, H.Z., Li, H.B., Jiang, Y. & Chen, F. * (2005). Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection. Analytical and Bioanalytical Chemistry, 383, 1014-1017.

135. He, H.Z., Li, H.B. & Chen, F. * (2005). Determination of vitamin B1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection. Analytical and Bioanalytical Chemistry, 383, 875-879.

134. Li, H.B. & Chen, F. * (2005). Preparative isolation and purification of phillyrin from the medicinal plant Forsythia suspensa by high-speed counter-current chromatography. Journal of Chromatography A, 1083, 102-105.

133. Ip, P.F. & Chen, F. * (2005). Employment of reactive oxygen species to enhance astaxanthin formation in Chlorella zofingiensis in heterotrophic culture. Process Biochemistry, 40, 3491-3496.

132. Ip, P.F. & Chen, F. * (2005). Peroxynitrite and nitryl chloride enhance astaxanthin production by the green microalga Chlorella zofingiensis in heterotrophic culture. Process Biochemistry, 40, 3595-3599.

131. Li, H.B. & Chen, F. * (2005). Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography.  Journal of Chromatography A, 1074, 107-110.

130. Chen, F.*, Li, H.B., Wong, R.N.S.,  Ji, B. & Jiang, Y. (2005). Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography.  Journal of Chromatography A, 1064, 183-186.

129. Li, H.B. & Chen, F. * (2005). Simultaneous separation and purification of five coumarins from the Chinese medicinal herb Cnidium monnieri by high-speed counter-current chromatography.  Journal of Separation Science, 28, 268-272.

128. Ip, P.F. & Chen, F.* (2005). Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark.  Process Biochemistry, 40, 733-738.

127. Liu, X.J., Jiang, Y. & Chen, F.* (2005). Fatty acid profile of the edible filamentous cyanobacterium Nostoc flagelliforme at different temperatures and developmental stages in liquid suspension culture.  Process Biochemistry, 40, 371-377.

126. Li, H.B. & Chen, F.* (2004). Preparative isolation and purification of bergapten and imperatorin from the medicinal plant Cnidium monnieri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase. Journal of Chromatography A, 1061, 51-54.

125. Wang, S.B., Chen, F. *, Sommerfeld, M. & Hu, Q. (2004). Proteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae).  Planta, 220, 17-29.

124. Li, H.B., Jiang, Y. & Chen, F.* (2004). Separation methods used for Scutellaria baicalensis active components.  Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 812, 277-290 (invited review for the Annual Review Volume).

123. Lu, H.T., Jiang, Y., Li, H.B., Chen, F. & Wong, M.H. (2004). Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection.  Chromatographia, 60, 259-264.

122. Li, X.Z., Zhang, D.H., Chen, F., Ma, J., Dong, Y.H. & Zhang, L.H. (2004).  Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium.  International Journal of Systematic and Evolutionary Microbiology 54, 2131-2136.

121. Li, H.B. & Chen, F.* (2004). Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography. Journal of Chromatography A, 1052(1-2), 229-232.

120. Kumar, D.S.S., Cheung, H.Y., Lau, C.S., Chen, F. & Hyde, K.D. (2004). In vitro studies of endophytic fungi from Tripterygium wilfordii with anti-proliferative activity on human peripheral blood mononuclear cells. Journal of Ethnopharmacology, 94, 295-300.

119. Li, H.B. & Chen, F.* (2004). Preparative isolation and purification of chuanxiongzine from the medicinal plant Ligusticum chuanxiong by high-speed counter-current chromatography. Journal of Chromatography A, 1047, 249-253.

118. Lu, H.T., Li, H.B.  Chen, F., Wong M.H. & Jiang, Y. (2004). Determination of triphenyltin and its metabolite diphenyltin by high-performance liquid chromatography with UV detection.  Chromatographia, 60, 113-116.

117. Chen, F., Chan, K.H., Jiang, Y., Kao, R.Y.T., Lu, H.T., Fan, K.W., Cheng, V.C.C., Tsui, W.H.W., Lee, T.S.W., Peiris, J.S.M. & Yuen, K.Y. (2004).  In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds. Journal of Clinical Virology, 31(1), 69-75. (The most viewed in the journal in 2004).

116. Ip, P.F., Wong, K. H. & Chen, F.* (2004). Enhanced production of astaxanthin by the green microalga Chlorella zofingiensis in mixotrophic culture. Process Biochemistry, 39(11), 1761-1766.

115. Chen, F.*, Lu, H.T. & Jiang, Y. (2004). Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography. Journal of Chromatography A, 1040(2), 205-208.

114. Jiang, Y., Lu, H.T. & Chen, F.* (2004). Preparative purification of glycyrrhizin extracted from the root of liquorice using high-speed counter-current chromatography. Journal of Chromatography A, 1033(1), 183-186.

113. Jiang, Y., Fan, K.W., Wong, R.T.Y. & Chen, F.* (2004). Fatty acid composition and squalene content of the marine microalga Schizochytrium mangrovei.  Journal of Agricultural and Food Chemistry, 52, 1196-1200.

112. Lu, H.T., Jiang, Y. & Chen, F.* (2004). Determination of squalene using high-performance liquid chromatography with diode array detection.  Chromatographia, 59, 367-371.

111. Wang, S.B., Hu, Q., Sommerfeld, M. & Chen, F. * (2004). Cell wall proteomics of the green alga Haematococcus pluvialis (Chlorophyceae).  Proteomics, 4, 692-708.

110. Liang, S.Z., Liu, X.M., Chen, F. & Chen, Z.J. (2004). Current microalgal health food R & D activities in China.  Hydrobiologia, 512, 45-48.

109. Lai, J.P., Jiang, Y., He, X.W., Huang, J.C. & Chen, F.* (2004).  Separation and determination of astaxanthin from microalgal and yeast samples by molecularly imprinted microspheres.  Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences , 804(1), 25-30.

108. Lu, H.T., Jiang, Y. & Chen, F.* (2004). Application of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Lonicerae.  Journal of Chomatography A, 1026(1-2), 185-190.

107. Lu, H.T., Jiang, Y. & Chen, F. (2004). Preparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon.  Journal of Chromatography A, 1023(1), 159-163.

106. Liu, X.J. & Chen, F. * (2003). Cell differentiation and colony alteration of Nostoc flagelliforme, an edible terrestrial cyanobacterium,in different liquid suspension cultures. Folia Microbiologica, 48 (5), 619-626.

105. Wang, S.B., Hu, Q., Sommerfeld, M. & Chen, F.* (2003). An optimized protocol for isolation of soluble proteins from microalgae by two-dimensional gel electrophoresis analysis. Journal of Applied Phycology, 15, 485-496.

104. Lu, H.T., Jiang, Y. & Chen, F. (2003). Application of high-speed counter-current chromatography to the preparative separation and purification of baicalin from the Chinese medicinal plant Scutellaria baicalensis.  Journal of Chromatography A, 1017,117-123.

103. Lai, J.P., He, X.W. & Chen, F. * (2003). Separation and determination of the antitumor drug piritrexim by molecularly imprinted microspheres in high performance liquid chromatography.  Analytical and Bioanalytical Chemistry, 377(1), 208-213.

102. Liu, X.J., Chen, F.* & Jiang, Y. (2003). Differentiation of Nostoc flagelliforme and its neighboring species using fatty acid profiling as a chemotaxonomic tool. Current Microbiology, 47 (6), 467-474.

101. Wen, Z.Y. & Chen, F. * (2003). Heterotrophic production of eicosapentaenoic acid by microalgae.  Biotechnology Advances, 21(4), 273-294. [Invited Article].

100. Li, X.Z., Dong, X.L., Zhao, C.X., Chen, Z. & Chen, F. (2003). Isolation and some properties of cellulose-degrading Vibrio sp. LX-3 with agar-liquefying ability from soil.  World Journal of Microbiology & Biotechnology, 19, 375-379.

99. Lu, H.T., Jiang, Y. & Chen, F. * (2003). Preparative separation and purification of squalene from the microalga Thraustochytrium sp. by high-speed counter-current chromatography.  Journal of Chromatography A, 994, 37-43.

98. Lai, J.P., He, X.W., Jiang, Y. & Chen, F. * (2003). Preparative separation and determination of matrine from the Chinese medicinal plant Sophora flavescens Ait by molecularly imprinted solid phase extraction. Analytical and Bioanalytical Chemistry, 375, 264-269.

97. Wen, Z.Y. & Chen, F. * (2002). Perfusion culture of the diatom Nitzschia laevis for ultra-high yield production of eicosapentaenoic acid.  Process Biochemistry, 38, 523-529.

96. Zhong, J.J., Pan, Z.W., Wang, Z.Y., Wu, J.Y., Chen, F., Takagi, M. & T. Yoshida (2002). Effect of mixing time on taxoid production in suspension cultures of Taxus chinensis in a centrifugal impeller bioreactor. Journal of Bioscience and Bioengineering, 94 (3), 244-250.

95. Shi, X.M., Jiang, Y. & Chen, F. * (2002).  High-yield production of lutein by the green microalga Chlorella protothecoides in heterotrophic fed-batch culture.  Biotechnology Progress, 18, 723-727.

94. Wen, Z.Y., Jiang, Y. & Chen, F. * (2002).  High cell density culture of the diatom Nitzschia laevis for eicosapentaenoic acid production: fed-batch development. Process Biochemistry, 37, 1447-1453.

93. Li, H.B., Jiang, Y. & Chen, F. * (2002).  Isolation and purification of lutein from the microalga Chlorella vulgaris by extraction after separation.  Journal of Agricultural and Food Chemistry, 50, 1070-1072.

92. Li, H.B., Lai, J.P., Jiang, Y. & Chen, F. * (2002).  Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography.  Journal of Chromatography A, 943(2), 235-239.

91. Wen, Z.Y. & Chen, F. * (2002). Continuous cultivation of the diatom Nitzschia laevis for eicosapentaenoic acid production: physiological study and process optimization.  Biotechnology Progress, 18, 21-28.

90. Yuan, J.P., Chen, F.*, Liu, X. & Li, X.Z. (2002).  Carotenoid composition in the green microalga Chlorococcum.  Food Chemistry, 76(3), 319-325.

89. Li, H.B. & Chen, F. * (2001). Preparative isolation and purification of salidroside from the Chinese medicinal plant Rhodiola sacchalinensis by high-speed counter-current chromatography. Journal of Chromatography A, 932, 91-95.

88. Fan, W.K., Chen, F., Jones E.B.G. & Vrijmoed L.L.P. (2001). Eicosapentaenoic acid and docosahexaenoic acid production and okara uilizing potential of thraustochytrids. Journal of Industrial Microbiology & Biotechnology. 27(4), 199-202.

87. Wen, Z.Y. & Chen, F. * (2001). A perfusion-cell bleeding strategy for enhancing the productivity of eicosapentaenoic acid in the culture of Nitzschia laevis. Applied Microbiology and Biotechnology, 57, 316-322.

86. Wen, Z.Y. & Chen, F. * (2001). Optimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis. Enzyme and Microbial Technology, 29, 341-347.

85. Wen, Z.Y. & Chen, F. * (2001).  Application of statistically-based experimental designs for the optimization of eicosapentaenoic acid production by the diatom Nitzschia laevis.  Biotechnology and Bioengineering, 75 (2), 159-169.

84. Li, H.B. & Chen, F. * (2001). Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection. Chromatographia, 54, 270-273.

83. Li, H.B. & Chen, F. * (2001). Preparative isolation and purification of six diterpenoids from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography.  Journal of Chromatography A, 925, 109-114.

82. Li, H.B. & Chen, F. * (2001). Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatogaphy.  Journal of Chromatography A, 925, 133-137.

81. Yuan, J.P. & Chen, F.* (2001). Indirect photometric ion chromatographic analysis of anions in Haematococcus pluvialis culture media.  Biotechnology Letters, 23(10), 757-760.

80. Ma, R.Y.N. & Chen, F. * (2001). Enhanced production of free trans-astaxanthin by oxidative stress in the cultures of the green microalga Chlorococcum sp.  Process Biochemistry, 36(12), 1175-1179.

79. Li, H.B., Chen, F. * & Xu, X.R. (2001).  Determination of iodide in seawater and urine by size exclusion chromatography with iodine-starch complex.  Journal of Chromatography A, 918, 335-339.

78. Li, H.B. & Chen, F. * (2001). Simultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography with diode array detection. Journal of Separation Science, 24, 271-274.

77. Yuan, J.P. & Chen, F. * (2001).  Kinetics for the reversible isomerization reaction of trans-astaxanthin. Food Chemistry, 73(2), 131-137.

76. Ma, R.Y.N. & Chen, F. * (2001). Induction of astaxanthin formation by reactive oxygen species in mixotrophic culture of Chlorococcum sp. Biotechnology Letters, 23(7), 519-523.

75. Li, H.B., Chen, F. *, Zhang, T.Y., Yang, F.Q. & Xu, G.Q. (2001). Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography.  Journal of Chromatography A, 905, 151-155. 

74. Li, H.B. & Chen, F. * (2000). Determination of viatmin B12 in pharmaceutical preparations by a highly sensitive fluorimetric method. Fresenius Journal of Analytical Chemistry (changed to Analytical and Bioanalytical Chemistry), 368(8), 836-838.

73. Fan, K.W., Chen, F., Jones, E.B.G. & Vrijmoed, L.L.P. (2000). Utilization of food processing waste by Thraustochytrids. Fungal Diversity, 5, 185-194.

72. Wen, Z.Y. & Chen, F. * (2000). Heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis: effects of silicate and glucose. Journal of Industrial Microbiology and Biotechnology, 25, 218-224.

71. Chen, H., Chen, F. *, Chiu, F.C.K. & Lo, C.M.Y. (2001).  The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza. Enzyme and Microbiology Technology, 28, 100-105.

70. Vonshak, A., Cheung, S.M. & Chen, F. (2000). Mixotrophic growth modifies the response of Spirulina (Arthrospira) platensis (cyanobacteria) cells to light.  Journal of Phycology, 36, 675-679.

69. Li, H.B. &  Chen, F. * (2000). A highly sensitive fluorimetric method for the determination of fluoride in biological material with Al 3+-calcein complex. Fresenius Journal of Analytical Chemistry (changed to Analytical and Bioanalytical Chemistry), 5, 501-504.

68. Li, H.B., Chen, F. * & Xu, X.R. (2000).  A highly sensitive spectrophotometric method with solid phase extraction for the determination of methylmercury in human hair. Journal of Analytical Toxicology, 24(8), 704-707.

67. Li, H.B., Chen, F. * & Jiang, Y. (2000). Determination of vitamin B12 in multivitamin tablets and fermentation medium by high performance liquid chromatography with fluorescence detection, Journal of Chromatography A, 891(2), 243-247.

66. Jiang, Y. & Chen, F. * (2000). Effects of temperature and temperature shift on docosahexaenoic acid production by the marine microalga Crypthecodinium cohnii.  Journal of the American Oil Chemists Society, 77, 613-617.

65. Jiang, Y. & Chen, F. * (2000). Effects of medium glucose concentration and pH on docosahexaenoic acid content of heterotrophic Crypthecodinium cohnii. Process Biochemistry, 35, 1205-1209.

64. Shi, X.M., Zhang, X.W. & Chen, F. * (2000).  Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources.  Enzyme and Microbial Technology, 27, 312-318.

63. Chen, H. & Chen, F. * (2000). Induction of phytoalexin formation in crown gall and hairy root cultures of Salvia miltiorrhiza by methyl viologen.  Biotechnology Letters, 22(8), 715-720.

62. Wen, Z.Y. & Chen, F. * (2000). Production potential of eicosapentaenoic acid by the diatom Nitzschia laevis.  Biotechnology Letters, 22(9), 727-733.

61. Li, H.B. & Chen, F. * (2000). Determination of silicate in water by ion exclusion chromatograpghy with conductivity detection. Journal of Chromatography A, 874(1), 143-147.

60. Li, H.B., Chen, F. * & Xu, X.R. (2000). Fluorimetric determination of methylmercury as an ion-association complex with rhodamine B in the presence of iodide.  Fresenius Journal of Analytical Chemistry(changed to Analytical and Bioanalytical Chemistry), 367(5), 499-501.

59. Wen, Z.Y., Teng, X.W. & Chen, F. * (2000). A novel perfusion system for hybridoma cell cultures by two step sequential sedimentation.  Journal of Biotechnology, 79, 1-11.

58. Chen, H. & Chen, F. * (2000). Effects of yeast elicitor on the growth and secondary metabolism of a high-tanshinone-producing line of the Ti transformed Salvia miltiorrhiza cells in suspension culture.  Process Biochemistry, 35, 837-840.

57. Zhang, X.W., Shi, X.M. & Chen, F. * (1999). A kinetic model for lutein production by the green microalga Chlorella protothecoides in heterotrophic culture.  Journal of Industrial Microbiology & Biotechnology, 23(6), 503-507.

56. Chen, H. & Chen, F. * (2000). Effect of yeast elicitor on the secondary metabolism of Ti transformed Salvia miltiorrhiza cell suspension cultures.  Plant Cell Reports, 19(7), 710-717.

55. Zhong, J.J., Chen, F. & Hu, W.W. (2000). High density cultivation of Panax notoginseng cells in stirred bioreactors for the production of ginseng biomass and ginseng saponin.  Process Biochemistry, 35, 491-496.

54. Yuan, J.P. & Chen, F. * (2000). Purification of trans-astaxanthin from a high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis.  Food Chemistry, 68, 443-448.

53. Jiang, Y. & Chen, F. * (1999). Effects of salinity on cell growth and docosahexaenoic acid content of the heterotrophic marine microalga Crypthecodinium cohnii.  Journal of Industrial Microbiology & Biotechnology, 23(6), 508-513.

52. Ren, G. & Chen, F. * (2000). Drying of steamed Asian ginseng (Panax ginseng) roots by microwave-hot air combination.  Pharmazie, 55(2), 124-128.

51. Ren, G., Zhang, X.W. & Chen, F. * (2000). Predicting colour kinetics during red Asian ginseng (Panax ginseng) preparation. Pharmazie, 55(4), 300-302.

50. Chen, Q., Chen, F. & Li, Z.Y. (1999).  Preparation and surfactant properties of amino acid based surfactants.  Chinese Chemical Letters, 10(9), 789-792.

49. Zhang X.W., Chen, F. * & Johns, M.R. (1999). Kinetic models for heterotrophic growth of Chlamydomonas reinhardtii in batch and fed-batch cultures. Process Biochemistry, 35, 385-389.

48. Yuan, J.P. & Chen, F. * (1999). Isomerization of trans-astaxanthin in organic solvents. Journal of Agricultural & Food Chemistry, 47, 3656-3660. 

47. Zhang, X.W., Gong, X.D. & Chen, F. * (1999). Dynamics and stability analysis of the growth and astaxanthin production system of Haematococcus pluvialis.  Journal of Industrial Microbiology & Biotechnology, 23(2), 133-137.

46. Chen, H., Chen, F. *, Zhang, Y.L. & Song, J.Y. (1999). Production of rosmarinic acid and lithospermic acid B in Ti transformed Salvia miltiorrhiza cell suspension cultures.  Process Biochemistry, 34, 777-784.

45. Jiang, Y., Chen, F. * & Liang, S.Z. (1999). Production potential of docosahexaenoic acid by the heterotrophic marine dinoflagellate Crypthecodinium cohnii.  Process Biochemistry, 34, 633-637.

44. Chen, H. & Chen, F. * (1999). Effects of methyl jasmonate and salicylic acid on cell growth and cryptotanshinone formation in Ti transformed Salvia miltiorrhiza cell suspension cultures.  Biotechnology Letters, 21(9), 803-807.

43. Zhang, Y.M. & Chen, F. * (1999). A simple method for efficient separation and purification of c-phycocyanin and allophycocyanin from Spirulina platensis. Biotechnology Techniques(changed to Biotechnology Letters), 13(9), 601-603.

42. Chen, H. & Chen, F. * (1999).  Kinetics of cell growth and secondary metabolism of a high-tanshinone-producing line of the Ti transformed Salvia miltiorrhiza cells in suspension cultures.  Biotechnology Letters, 21(8), 701-705.

41. Zhang, X.W., Gong, X.D. & Chen, F.*  (1999). Kinetic models for astaxanthin production by high cell density mixotrophic culture of the microalga Haematococcus pluvialis. Journal of Industrial Microbiology Biotechnology, 23, 691-696.

40. Boussiba, S., Wang, B., Yuan, J.P., Zarka. A. & Chen, F.* (1999). Changes in pigment profile in the green alga Haematococcus pluvialis exposed to environmental stresses.  Biotechnology Letters, 21, 601-604.

39. Zhang, X.W., Zhang, Y.M. & Chen, F.*  (1999). Application of mathematical models to the determination optimal glucose concentration and light intensity for mixotrophic culture of Spirulina platensis.  Process Biochemistry, 34(5), 477-481.

38. Shi, X.M., Liu, H.J., Zhang, X.W. & Chen, F.* (1999). Production of biomass and lutein by Chlorella protothecoides at various glucose concentrations in heterotrophic cultures. Process Biochemistry, 34(4), 341-347.

37. Ren, G. & Chen, F. * (1999). Simultaneous quantification of ginsenosides in American ginseng (Panax quinquefolium) root powder by visible/near infrared reflectance spectroscopy.  Journal of Agricultual & Food Chemistry, 47, 2771-2775.

36. Chen, H., Chen, F. *, Zhang, Y.L. & Song, J.Y. (1999). Production of lithospermic acid B and rosmarinic acid in hairy root cultures of Salvia miltiorrhiza.  Journal of Industrial Microbiology & Biotechnology, 22, 133-138.

35. Ren, G. & Chen, F. * (1999). Degradation of ginsenosides in American ginseng (Panax quiquefolium) extracts during microwave and conventional heating.  Journal of Agricultural & Food Chemistry, 47(4), 1501-1505.

34. Shi, X.M. & Chen, F. * (1999). Production and rapid extraction of lutein and the other lipid-soluable pigments from Chlorella protothecoides grown under heterotrophic and mixotrophic conditions.  Nahrung/Food, 43(2), 109-113.

33. Yuan, J.P. & Chen, F. * (1999).  Simultaneous separation and determination of sugars, ascorbic acid and furanic compounds by HPLC-dual detection.  Food Chemistry, 64(3), 423-427.

32. Yuan, J.P. & Chen, F. * (1999). Hydrolysis kinetics of astaxanthin esters and stability of astaxanthin of Haematococcus pluvialis during saponification. Journal of Agricultural & Food Chemistry, 47(1), 31-35.

31. Zhang, X.W., Zhang, Y.M. & Chen, F.* (1998). Kinetic models for phycocyanin production by high cell density mixotrophic culture of the microalga Spirulina platensis.  Journal of Industrial Microbiology & Biotechnology, 21(6), 283-388.

30. Yuan, J.P. & Chen, F. * (1998). Degradation of ascorbic acid in aqueous solution.  Journal of Agricultural & Food Chemistry, 46(12), 5078-5082.

29. Vazhappilly, R. & Chen, F. * (1998). Heterotrophic production potential of omega-3 polyunsaturated fatty acids by microalgae and algae-like microorganisms.  Botanica Marina, 41, 553-558.

28. Yuan, J.P. & Chen, F. * (1998).  Chromatographic separation and purification of trans-astaxanthin from the extracts of Haematococcus pluvialis.  Journal of Agricultural & Food Chemistry, 46(8), 3371-3375.

27. Li, X., Lin, W., Gao, P. & Chen, F.  (1998). Endoglucanase S, a novel endocellulase exhibiting exoglucanase activity from a newly isolated Streptomyces sp. LX. Journal of Applied Microbiology, 85, 347-356.

26. Yuan, J.P., Chen, H. & Chen, F. * (1998). Simultaneous determination of rosmarinic acid, lithospermic acid B and related phenolics in Salvia miltiorrhiza by HPLC.  Journal of Agricultural & Food Chemistry, 46(7), 2651-2654.

25. Li, X. & Chen, F. * (1998). Improvement in isolation of gliding bacteria by the elimination of soluable sugars.  Biotechnology Techniques(changed to Biotechnology Letters), 12(5), 343-345.

24. Ren, G. & Chen, F. * (1998). Drying of American ginseng (Panax quinquefolium) roots by microwave-hot air combination.  Journal of Food Engineering, 35(4), 433-443.

23. Gong, X. & Chen, F. * (1998). Influence of medium components on astaxanthin content and production of Haematococcus pluvialis.  Process Biochemistry, 33(4), 385-391.

22. Yuan, J.P. & Chen, F. * (1998). Separation and identification of furanic compounds in fruit juices and drinks by HPLC-PDA detection.  Journal of Agricultural & Food Chemistry, 46, 1286-1291.

21. Vazhappilly, R. & Chen, F. * (1998). Eicosapentaenoic acid and docosahexaenoic acid production potential of microalgae and their heterotrophic growth.  Journal of the American Oil Chemists Society, 75, 393-397.

20. Chen, H., Yuan, J.P., Chen, F. *, Zhang, Y.L. & Song, J.Y. (1997). Tanshinone production in Ti transformed Salvia miltiorrhiza cell suspension cultures.  Journal of Biotechnology, 58(3), 147-156.

19. Chen, F. *, Chen, H. & Gong, X. (1997). Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behaviour of the cell suspensions.  Bioresource Technology, 62(1-2), 19-24.

18. Gong, X.D. & Chen, F. * (1997).  Optimization of culture medium for growth of Haematococcus pluvialis.  Journal of Applied Phycology, 9(5), 437-444.

17.  Shi, X.M., Chen, F. *, Yuan, J.P. & Chen, H. (1997).  Heterotrophic production of lutein by selected Chlorella strains.  Journal of Applied Phycology, 9(5), 445-450.

16. Ren, G., Chen, F. *, Lian, H., Zhao, J. and Gao, X. (1997). Changes in hormone contents of Panax quinquefolium seeds during stratification. Journal of Horticultural Science (changed to Journal of Horticultural Science & Biotechnology) , 72(6), 901-906.

15. Gong, X.D. & Chen, F. * (1997). Rapid detection of heterotrophic growth of Haematococcus pluvialis using indirect conductimetry.  Biotechnology Techniques(changed to Biotechnology Letters), 11(11), 841-844.

14. Ren, G. and Chen, F. * (1997).  Determination of moisture content of ginseng by near infra-red reflectance spectroscopy.  Food Chemistry, 60, 433-436.

13. Yuan, J.P. & Chen, F. * (1997). Identification of astaxanthin isomers in Haematococcus lacustris by HPLC-photodiode array detection.  Biotechnology Techniques(changed to Biotechnology Letters), 11(7), 455-459.

12. Yuan, J.P., Gong, X.D. & Chen, F. * (1997).  Separation and analysis of carotenoids and chlorophylls in Haematococcus lacustris by high-performance liquid chromatography photodiode array detection.  Journal of Agricultural & Food Chemistry, 45(5), 1952-1956.

11. Chen, F. * & Zhang, Y. (1997).  High cell density mixotrophic culture of Spirulina platensis on glucose for phycocyanin production using a fed-batch system.  Enzyme and Microbial Technology, 20, 221-224.

10. Shi, X.M. and Chen, F. * (1997). Stability of lutein under various storage conditions.  Nahrung/Food, 41(1), 38-41.

9. Chen, F.* (1996).  High cell density culture of microalgae in heterotrophic growth.  Trends in Biotechnology, 14(11), 421-426 [featured in the cover of the journal; the article was also cited by a landmark article in Science (292: 2073-2075, 2001) as one of the major reasons they initiated the study].

8. Chen, F. *, Zhang, Y. & Guo, S. (1996).  Growth and phycocyanin formation of Spirulina platensis in photoheterotrophic culture.  Biotechnology Letters, 18(5), 603-608.

7. Chen, F. * & Johns, M.R. (1996). Heterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture.  Process Biochemistry, 31(6), 601-604.

6. Chen, F. & Johns, M.R. (1996).  High cell density culture of Chlamydomonas reinhardtii on acetate using fed-batch and hollow-fibre cell-recycle systems.  Bioresource Technology, 55(2), 103-110.

5. Chen, F. * & Johns, M.R. (1996). Relationship between substrate inhibition and maintenance energy of Chlamydomonas reinhardtii in heterotrophic culture.  Journal of Applied Phycology, 8(1), 15-19.

4. Yuan, J.P., Gong X.-D. & Chen, F. * (1996). Separation and identification of astaxanthin esters and chlorophylls in Haematococcus lacustris by HPLC.  Biotechnology Techniques(changed to Biotechnology Letters), 10(9), 655-660.

You are the visitor of this page since 1st March, 2001