School of Biological Sciences
The University of Hong Kong
Professor M. L. Chye
Wilson and Amelia Wong Professor in Plant Biotechnology
Tel. +852 2299-0319
M.L. Chye, the Wilson and Amelia Wong Professor in Plant Biotechnology at HKU, completed her PhD on a Commonwealth Scholarship at the University of Melbourne and received postdoctoral training in Plant Molecular Biology at the Rockefeller University (New York) and the Institute of Molecular and Cell Biology (Singapore). She joined the University of Hong Kong in 1993 and was promoted to Professor in 2005. Her administrative roles have included being an Associate Dean (April 2010-April 2016) and Dean (May 2016-August 2019) of the Graduate School.
Listed as a top 1% cited scholar at HKU 2013-2020 (http://hub.hku.hk/local/top1pc/top1pc.jsp), her research awards include an Edward Clarence Dyason Universitas 21 Fellowship (2004/05), an Outstanding University Researcher Award (2006/07), a Croucher Senior Research Fellowship (2007/08), and an Eileen Mary Harris Scholarship (2013). She currently serves as an Associate Editor for Planta (Springer) and Plant Molecular Biology (Springer), and was Chair of the 4th Asian Symposium on Plant Lipids (2011) and the 12th International Symposium on Biocatalysis and Agricultural Biotechnology (ISBAB) in 2016. She works with current international research collaborators from Australia, Canada, China, France, and the UK, as well as local collaborators in two AoEs (AoE/M-05/12 and AoE/M-403/16).
Laboratory of Plant Molecular Biology
The main focus of the Chye Lab is to understand the function and mechanism of action of stress-induced plant proteins, particularly plant acyl-CoA-binding proteins (ACBPs). We intend to use them to generate transformed plants that can better tolerate abiotic and biotic stresses since these stresses account for ~40 % loss in crop productivity. Ultimately, investigations on plant ACBPs, and others, will be applied to agriculture and phytoremediation.
Our projects are supported by the Wilson and Amelia Wong Endowment Fund, Research Grants Council of Hong Kong, NSFC/RGC Joint Research Scheme, “Centre for Organelle Biogenesis and Function” Area of Excellence AoE/M-05/12 (http://www.cuhk.edu.hk/centre/iCell/), “Centre for Genomic Studies on Plant-Environment Interaction for Sustainable Agriculture and Food Security” Area of Excellence AoE/M-403/16” (https://aoegspei.cuhk.edu.hk/aboutus/) and the State Key Laboratory of Agrobiotechnology (CUHK).
Arabidopsis acyl-CoA-binding proteins (ACBPs) and stress tolerance
We are studying a family of plant ACBPs which bind acyl-CoA esters and transport them within the plant cell. In Arabidopsis and rice, six genes encode four structurally distinct classes of ACBPs (Leung et al., 2004; Xiao and Chye, 2009; 2011b; Meng et al., 2010; 2014). In Arabidopsis the six members are
In our attempt to understand the function of Arabidopsis ACBPs, we have identified the amino acid residues in the acyl-CoA-binding domain that are important in binding acyl-CoA esters (Chye et al., 2000; Leung et al., 2004; 2006). Some ACBPs can also bind phospholipids (Chen et al., 2008; Du et al., 2010; Chen et al., 2010; Xiao et al., 2010). On lipid analysis, Arabidopsis acbp mutants and transgenic Arabidopsis lines overexpressing ACBPs showed alterations in lipid composition (Xiao et al., 2008b; 2010; Chen et al., 2008; 2010; Du et al., 2010, 2013a, 2013b). ACBPs with ankyrin repeats (Li and Chye, 2004; Gao et al., 2009; 2010; Du et al., 2013b) and kelch motifs (Leung et al., 2004; Li et al., 2008; Xiao et al., 2008b) have been demonstrated to mediate protein-protein interactions.
We have observed that certain ACBPs are induced by various forms of abiotic and biotic stresses (Xiao et al., 2008a; Li et al., 2008; Chen et al., 2008; Gao et al., 2009; 2010; Xiao and Chye; 2010, 2011a; Zheng et al., 2012; Du et al., 2013a, 2013b). Subsequently, when these ACBPs were overexpressed in transgenic plants, the resultant lines were conferred stress tolerance. ACBP6-overexpressors were freezing tolerant (Chen et al., 2008; Liao et al., 2014; US Patent No. 8378172) and ACBP2-overexpressors were drought tolerant (Du et al., 2013b; US Patent Application 13,667,569). ACBP1- and ACBP2-overexpressors also displayed tolerance to heavy metal and oxidative stresses (Xiao et al., 2008; Gao et al., 2009; 2010; Du et al., 2014). Interestingly, ACBP1-overexpressors accumulate Pb(II) in shoots making ACBP1 applicable for phytoremediation, a low-cost solar-driven process that removes pollutants from the environment in situ (Xiao et al., 2008a, Xiao and Chye, 2008; US Patent No. 7,880,053).
Stress-inducible HMGS, an enzyme in plant isoprenoid metabolism
We are investigating the role of 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) in plant isoprenoid metabolism. HMGS is an enzyme in the cytosolic mevalonate pathway which produces sterols, sesquiterpenes and polyterpenes. Its expression is stress-inducible and is highest during early development in flower, seed and seedling (Alex et al., 1999). Four isogenes encoding HMGS are differentially expressed in Brassica juncea (Nagegowda et al., 2005).
Using green fluorescent protein fusions, B. juncea HMGS1 (BjHMGS) has been subcellularly localized to the cytosol (Nagegowda et al., 2005). We have biochemically purified and characterized His-tagged recombinant BjHMGS expressed in Escherichia coli, presenting a first detailed characterization of a plant HMGS, and the amino acids involved in catalysis were identified by site-directed mutagenesis (Nagegowda et al., 2004). In collaboration with the Bach Lab and the Noel Lab, the crystal structure of Brassica HMGS covalently bound to specific inhibitor F-244 has been determined, providing a first approach towards the design of more potent cholesterol-lowering drugs and antibiotics that target the mevalonate pathway (Pojer et al., 2006).
When mutant and wild-type BjHMGS were expressed in transgenic Arabidopsis, the genes of the sterol biosynthetic pathway were upregulated resulting in an overaccumulation of phytosterols (stimasterols, campesterol and stigmasterols), and the HMGS-overexpressing lines were better protected against oxidative stress and Botrytis infection (Wang et al., 2012). Furthermore, the overexpression of HMGS variant S359A in a model plant from the family Solanaceae, not only resulted in phytosterol accumulation but showed enhanced plant growth, pod size and seed yield (Liao et al., 2014; US Patent Application 14/260,561). The potential of S359A in boosting seed yield may now be tested in food crops.
HKU Press releases:
HKU plant scientists identify a new strategy to enhance rice grain yield (1/12/19)
HKU researchers generate tomatoes with enhanced antioxidant properties by genetic engineering (9/11/17)
Enhancing drought tolerance in plants:
Nikkei Asia Review (13/1/16) http://asia.nikkei.com/Tech-Science/Science/Hong-Kong-scientists-engineer-plants-for-a-warmer-planet
HKU scientists discover a drought tolerance gene that may help plants survive global warming (22/11/15):
HKU identifies a new strategy to protect flowers from freezing stress (9/6/14):
- S Panthapulakkal Narayanan, SC Lung, P Liao, C Lo and ML Chye. 2020. The overexpression of OsACBP5 protects transgenic rice against necrotrophic, hemibiotrophic and biotrophic pathogens. Sci. Rep. 10: 14918. https://doi.org/10.1038/s41598-020-71851-9
- J Jing, ZH Guo, Q Hao and ML Chye. Crystal structure of the rice acyl-CoA-binding protein OsACBP2 in complex with C18:3-CoA reveals a novel binding pattern of binding to acyl-CoA esters. FEBS Letts. https://doi.org/10.1002/1873-3468.13923
- JA Aznar-Moreno, M Venegas-Calerón, Z Du, R Garcés, JA Tanner, ML Chye, E Martínez-Force and JJ Salas. 2020. Characterization and function of a sunflower (Helianthus annuus L.) Class II acyl-CoA-binding protein. Plant Sci. 300: 110630 https://doi.org/10.1016/j.plantsci.2020.110630
- P Liao, KP Leung, SC Lung, S Panthapulakkal Narayanan, L Jiang and ML Chye. 2020. Subcellular localization of rice acyl-CoA-binding proteins ACBP4 and ACBP5 supports their non-redundant roles in lipid metabolism. Front. Plant Sci. 11:331. https://doi.org/10.3389/fpls.2020.00331
- N Amiruddin, PL Chan, N Azizi, PE Morris, KL Chan, PW Ong, R Rosli, SS Masura, DJ Murphy, R Sambanthamurthi, RP Haslam, ML Chye, JL Harwood and ETL Low. 2020. Characterisation of oil palm acyl-CoA-binding proteins and correlation of their gene expression with oil synthesis. Plant Cell Physiol. 61: 735-745. https://doi.org/10.1093/pcp/pcz237
- Y Zhou, WJ Tan, LJ Xie, H Qi, YC Yang, LP Huang, YX Lai, YF Tan, DM Zhou, LJ Yu, QF Chen, ML Chye and S Xiao. 2020. Polyunsaturated linolenoyl‐CoA modulates ERF‐VII‐mediated hypoxia signaling in Arabidopsis. Journal of Integrative Plant Biology 3: 330-348, https://doi.org/10.1111/jipb.12875
- ZH Guo, RP Haslam, LV Michaelson, EC Yeung, SC Lung, JA Napier and ML Chye. 2019. The overexpression of rice ACYL-COA-BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice. Plant Journal 100: 1132-1147, https://doi.org/10.1111/tpj.14503
- P Liao, HK Woodfield, JL Harwood, ML Chye and S Scofield. 2019. Comparative transcriptomics analysis of Brassica napus L. during seed maturation reveals dynamic changes in gene expression between embryos and seed coats and distinct expression profiles of acyl-CoA-binding proteins for lipid accumulation. Plant Cell Physiol. 60: 2812–2825, https://academic.oup.com/pcp/advance-article/doi/10.1093/pcp/pcz169/5555773
- P Liao, SC Lung, W L Chan, TJ Bach, C Lo and ML Chye. 2019. Overexpression of HMG-CoA synthase promotes Arabidopsis root growth and adversely affects glucosinolate biosynthesis. J. Exp. Botany 71: 272-287, https://doi.org/10.1093/jxb/erz420
- ZH Guo, ZW Ye, RP Haslam, LV Michaelson, JA Napier and ML Chye. 2019. Arabidopsis cytosolic acyl-CoA binding proteins function in determining seed oil content. Plant Direct 3: 1-9 e00182, DOI: 10.1002/pld3.182
- SP Narayanan, P Liao, PWJ Taylor, C Lo and ML Chye. 2019. Overexpression of a monocot acyl-CoA-binding protein confers broad-spectrum pathogen protection in a dicot. Proteomics https://doi.wiley.com/10.1002/pmic.201800368
- SC Lung, and ML Chye. 2019. Arabidopsis acyl-CoA-binding proteins regulate the synthesis of lipid signals. New Phytologist 223: 113-117, https://doi.org/10.1111/nph.15707
- R Miao, SC. Lung, X Li, XD Li, and ML Chye. 2019. Thermodynamic insights into an interaction between ACYL-COA-BINDING PROTEIN2 and LYSOPHOSPHOLIPASE2 in Arabidopsis. J. Biol. Chem. 294: 6214-6226, https://www.jbc.org/content/early/2019/02/19/jbc.RA118.006876.full.pdf
- MX Chen, TH Hu, FY Zhu, ZY Du, Y Xue, C Lo, ML Chye. 2018. Arabidopsis acyl-CoA-binding protein ACBP1 interacts with AREB1 and mediates salt and osmotic signaling in seed germination and seedling growth. Environ. Exp. Bot. 156: 130-140, DOI: 10.1016/j.envexpbot.2018.09.007
- SC Lung, P Liao, E Yeung, AS Hsiao, Y Xue, and ML Chye. 2018. Arabidopsis ACYL-COA-BINDING PROTEIN1 interacts with STEROL C4-METHYL OXIDASE1-2 to modulate gene expression of homeodomain-leucine zipper IV transcription factors. New Phytologist 218: 183–200, DOI: 10.1111/nph.14965
- TH Hu, SC Lung, ZW Ye and ML Chye. 2018. Depletion of Arabidopsis ACYL-COA-BINDING PROTEIN3 affects fatty acid composition in the phloem. Front. Plant Sci. 9:2. doi: 10.3389/fpls.2018.00002
- P Liao, X Chen, M Wang, TJ Bach and ML Chye. 2018. Improved fruit α-tocopherol, carotenoid, squalene and phytosterol content through manipulation of Brassica juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in transgenic tomato. Plant Biotechnol. J. 16: 784–796, DOI: 10.1111/pbi.12828
- SC Lung, P Liao, E Yeung, AS Hsiao, Y Xue, and ML Chye. 2017. Arabidopsis acyl-CoA-binding protein ACBP1 modulates sterol synthesis during embryogenesis. Plant Physiology 174: 1420-1435, DOI:10.1104/pp.17.00412
- ZH Guo, WH. Chan, GK. Kong, Q Hao and ML Chye. 2017. The first plant acyl-CoA-binding protein structures: the close homologues OsACBP1 and OsACBP2 from rice. Acta Cryst. D73: 438-448, DOI:10.1107/S2059798317004193
- ZW Ye, J Xu, J Shi, D Zhang and ML Chye. 2017. Kelch-motif containing acyl-CoA binding proteins AtACBP4 and AtACBP5 are differentially expressed and function in floral lipid metabolism. Plant Mol. Biol. 93: 209-225, DOI: 10.1007/s11103-016-0557-5
- ZW Ye, QF Chen and M-L. Chye. 2017. Arabidopsis thaliana acyl-CoA-binding protein ACBP6 interacts with plasmodesmata-located protein PDLP8. Plant Signaling & Behaviour 12:8, e1359365, DOI: 10.1080/15592324.2017.1359365 http://dx.doi.org/10.1080/15592324.2017.1359365
- ZY Du, T Arias, W Meng and ML Chye. 2016. Plant acyl-CoA-binding proteins: an emerging family in plant development and stress responses. Prog. Lipid Res 63: 165-181, DOI: 10.1016/j.plipres.2016.06.002
- ZW Ye, SC Lung, TH Hu, QF Chen, YL Suen, M Wang, S Hoffmann-Benning, E Yeung and ML Chye. 2016. Arabidopsis acyl-CoA-binding protein ACBP6 localizes in the phloem and affects jasmonate composition. Plant Mol. Biol. 92: 717–730, DOI: 10.1007/s11103-016-0541-0.
- Y Xue, SC Lung and ML Chye. 2016. Present status and future prospects of transgenic approaches for drought tolerance. In “Drought Tolerance in Plants, Vol 2: Molecular and Genetic Perspectives" (ISBN 978-3-319-32421-0) edited by LS Tran, DJ Burritt, MA Hossain, SH Wani and S Bhattacharjee. Springer, USA, Chapter 20, p. 549-569.
- P Liao, A Hemmerlin, TJ Bach and ML Chye. 2016. The potential of the mevalonate pathway for enhanced isoprenoid production. Biotechnology Advances 34: 697-713.
- SC Lung and ML Chye. 2016. Acyl-CoA-binding proteins in plant development. In “Lipids in plant and algae development” (ISBN 9783319259796) edited by Y Nakamura and Y Li-Beisson. Springer. Chapter 15, pp. 363-404. http://www.springer.com/us/book/9783319259772
- JA Aznar-Moreno, M Venegas-Caleron, ZY Du, R Garces, JA Tanner, ML Chye, E Martinez-Force and JJ Salas. 2016. Characterization of a small acyl-CoA-binding protein (ACBP) from Helianthus annuus L. and its binding affinities. Plant Physiology and Biochemistry 102: 141-150.
- SC Lung and ML Chye. 2016. Deciphering the roles of acyl-CoA-binding proteins in plant cells. Protoplasma 253: 1177-119, DOI 10.1007/s00709-015-0882-6.
- SC Lung and ML Chye. 2016. The binding versatility of plant acyl-CoA-binding proteins and their significance in lipid metabolism. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1861: 409-1421, Special Issue on Plant Lipid Biology 10.1016/j.bbalip.2015.12.018
- ZW Ye and ML Chye. 2016. Plant cytosolic acyl-CoA-binding proteins. Lipids 51: 1-13, 50th Commemorative Issue DOI 10.1007/s11745-015-4103-z
- ZY Du, MX Chen, QF Chen, JD Gu and ML Chye. 2015. Expression of Arabidopsis acyl-CoA-binding proteins AtACBP1 and AtACBP4 confers Pb(II) accumulation in Brassica juncea roots. Plant Cell Environ. 38: 101–117.
- AS Hsiao, RP Haslam, LV Michaelson, P Liao, QF Chen, S Sooriyaarachchi, SL Mowbray, JA Napier, JA Tanner and ML Chye. 2015. Arabidopsis cytosolic acyl-CoA-binding proteins ACBP4, ACBP5 and ACBP6 have overlapping but distinct roles in seed development. Bioscience Reports 34: 865–877.
- AS Hsiao, EC Yeung, ZW Ye and ML Chye. 2015. The Arabidopsis cytosolic acyl-CoA-binding proteins play combinatory roles in pollen development. Plant Cell Physiol. 56: 322-333.
- YL Yung, MY Cheung, R Miao, YH Fong, KP Li, MH Yu, ML Chye, KB Wong and HM Lam. 2015. Site-directed mutagenesis shows the significance of interactions with phospholipids and the G-protein OsYchF1 on the physiological functions of the rice GTPase-Activating Protein 1 (OsGAP1). J. Biol. Chem. 290: 23984-23996.
- YT Lai, YY Chang, L Hu, Y Yang, ZY Du, H Li, JA Tanner, ML Chye, C Qian and H Sun. 2015. Rapid labeling of intracellular His-tagged proteins in living cells. Proc. Natl Acad. Sci. USA 112: 2948-2953.
- W Meng, AS Hsiao, C Gao, L Jiang and ML Chye. 2014. The subcellular localization of rice ACBPs indicates that OsACBP6-GFP is targeted to the peroxisome. New Phytologist 203: 469-482.
- W Meng and ML Chye. 2014. Rice acyl-CoA-binding proteins OsACBP4 and OsACBP5 are differentially localized in the endoplasmic reticulum of transgenic Arabidopsis. Plant Signaling & Behavior 9:e29544.
- P Liao, QF Chen and ML Chye. 2014. Transgenic Arabidopsis flowers overexpressing acyl-CoA-binding protein ACBP6 are freezing tolerant. Plant Cell Physiol. 55: 1055-1071.
- P Liao, H Wang, M Wang, AS Hsiao, TJ Bach, ML Chye. 2014. Transgenic tobacco overexpressing Brassica juncea HMG-CoA synthase 1 shows increased plant growth, pod size and seed yield. PLOS ONE 9: e98264 (http://dx.plos.org/10.1371/journal.pone.0098264).
- P Liao, H Wang, A Hemmerlin, DA Nagegowda, TJ Bach, M Wang and ML Chye. 2014. Past achievements, current status and future perspectives of studies on 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) in the mevalonate (MVA) pathway. Plant Cell Reports 33: 1005-1022.
- MX Chen, SX Zheng, YN Yang,C Xu, Y Wang, JS Liu, WD Yang, ML Chye and HY Li. 2014. Strong seed-specific protein expression from the Vigna radiata storage protein 8SGα promoter in transgenic Arabidopsis seeds. Journal of Biotechnology 174: 49-56.
- MX Chen, SC Lung, ZY Du and ML Chye. 2014. Engineering plants to tolerate abiotic stresses. In a special issue on "Transformation methods and innovation platforms in plant biotechnology". Biocatalysis and Agricultural Biotechnology 3: 81-87, edited by I. Kalvochuk and R. Weselake. Elsevier.
- Y Xue, S Xiao, J Kim, SC Lung, L Chen, JA Tanner, MC Suh and ML Chye. 2014. Arabidopsis membrane-associated acyl-CoA-binding protein AtACBP1 is involved in stem cuticle formation. J. Exp. Bot. 18: 5473-5483.
- AS Hsiao, RP Haslam. LV Michaelson. P Liao, JA Napier and ML Chye. 2014. Gene expression in plant lipid metabolism in Arabidopsis seedlings. PLOS ONE 9:e107372 (http://dx.plos.org/10.1371/journal.pone.0107372)
- ZY Du, MX Chen, QF Chen, S Xiao and ML Chye. 2013a. Arabidopsis Acyl-CoA-Binding Protein ACBP1 participates in the regulation of seed germination and seedling development. Plant Journal 74: 294-309.
- ZY Du, MX Chen, QF Chen, S Xiao and ML Chye. 2013b. Overexpression of Arabidopsis Acyl-CoA-Binding Protein ACBP2 enhances drought tolerance. Plant Cell Environ. 36: 300-314.
- KW Kwan, ZW Ye, ML Chye and AHW Ngan. 2013. A mathematical model on water redistribution mechanism of the seismonastic movement of Mimosa pudica. Biophysical J. 105: 266-275
- MX Chen, YN Yang, SX Zheng, C Xu, Y Wang, JS Liu, WD Yang, ML Chye and HY Li. 2013. A Vigna radiata 8S globulin α’ promoter drives efficient expression of GUS in Arabidopsis cotyledonary embryos. J. Agri. Food Chem. 61: 6423–6429.
- ZY Du and ML Chye. 2013. Interactions between Arabidopsis acyl-CoA-binding proteins and protein partners. Planta 238: 239-245.
- F Zhu, L Li, PY Lam, MX Chen, ML Chye and C Lo. 2013. Sorghum extracellular leucine-rich repeat protein SbLRR2 mediates lead tolerance in transgenic Arabidopsis. Plant Cell Physiol. 54: 1549-1559.
- SX Zheng, S Xiao and ML Chye. 2012. The gene encoding Arabidopsis Acyl-CoA-Binding Protein 3 is pathogen-inducible and subject to circadian regulation. J. Exp. Bot. 63: 2985-3000.
- H Wang, DA Nagegowda, R Rawat, P Bouvier-Navé, D Guo, TJ Bach and ML Chye. 2012. Overexpression of Brassica juncea wild-type and mutant HMG-CoA synthase 1 in Arabidopsis up-regulates genes in sterol biosynthesis and enhances sterol production and stress tolerance. Plant Biotechnol. J. 10: 31-42.
- S Xiao and ML Chye. 2011a. Overexpression of Arabidopsis acyl-CoA-binding protein 3 enhances NPR1-dependent plant resistance to Pseudomonas syringae pv. tomato DC3000 Plant Physiology 156: 2069-2081.
- S Xiao and ML Chye. 2011b. New roles for acyl-CoA-binding proteins in plant development, stress responses and lipid metabolism Prog. Lipid Res. 50: 141-151.
- W Meng, YCF Su, RMK Saunders and ML Chye. 2011. The rice ACBP gene family: phylogeny, expression and functional analysis. New Phytologist 189: 1170-1184.
- S Xiao, W Gao, QF Chen, SW Chan, SX Zheng, J Ma, M Wang, R Welti and ML Chye. 2010. Overexpression of Arabidopsis acyl-CoA-binding protein ACBP3 promotes starvation-induced and age-dependent leaf senescence. Plant Cell 22: 1463-1482.
- W Gao, HY Li, S Xiao and ML Chye. 2010. Protein interactors of acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis. Plant Signaling & Behavior 5: 1025-1027.
- S Xiao and ML Chye. 2010. The Arabidopsis thaliana ACBP3 regulates leaf senescence by modulating phospholipid metabolism and ATG8 stability. Autophagy 6: 802-804
- W Gao, HY Li, S Xiao and ML Chye. 2010. Acyl-CoA-binding protein 2 binds lysophospholipase 2 and lysoPC to promote tolerance to cadmium-induced oxidative stress in transgenic Arabidopsis. Plant Journal 62: 989-1003.
- ZY Du, S Xiao, QF Chen and ML Chye. 2010. Arabidopsis acyl-CoA-binding proteins ACBP1 and ACBP2 show different roles in freezing stress. Plant Signaling & Behavior 5: 607-609.
- QF Chen, S Xiao, W Qi, G Mishra, J Ma, M Wang and ML Chye.2010. The Arabidopsis acbp1acbp2 double mutant lacking acyl-CoA-binding proteins ACBP1 and ACBP2 is embryo lethal. New Phytologist 186: 843-855.
"As simple as ACB – new insights into the role of acyl-CoA-binding proteins in Arabidopsis" JA Napier and RP Haslam. New Phytologist 186: 781-783.
- ZY Du, S Xiao, QF Chen and ML Chye.2010. Depletion of the membrane-associated acyl-CoA-binding protein ACBP1 confers freezing tolerance in Arabidopsis. Plant Physiology 152: 1585-1597.
- W Gao, S Xiao, HY Li, SW Tsao and ML Chye. 2009. Arabidopsis thaliana acyl-CoA-binding protein ACBP2 interacts with a heavy-metal-binding farnesylated protein AtFP6. New Phytologist 181: 89-102.
- HY Li and ML Chye. 2009. Use of green fluorescent protein to investigate expression of plant-derived vaccines. In “Viral applications of green fluorescent protein”, Methods in Molecular Biology 515: Chapter 19, pp. 275-287, edited by BA Hicks.
- S Xiao and ML Chye. 2009. An Arabidopsis family of six acyl-CoA-binding proteins has three cytosolic members. Plant Physiol Biochem 47: 479-484.
- S Xiao, QF Chen and ML Chye. 2009. Light-regulated Arabidopsis ACBP4 and ACBP5 encode cytosolic acyl-CoA-binding proteins that bind phosphatidylcholine and oleoyl-CoA ester. Plant Physiol Biochem 47: 926-933.
- S Xiao, QF Chen and ML Chye. 2009. Expression of ACBP4 and ACBP5 proteins is modulated by light in Arabidopsis. Plant Signaling & Behavior 4: 1063-1065.
- W Ubhayasekera, R Rawat, SWT Ho, M Wiweger, S Von Arnold, ML Chye and SL Mowbray. 2009. The first crystal structures of a family 19 class IV chitinase: the enzyme from Norway spruce. Plant Mol Biol 71: 277-289.
- S Xiao, W Gao, QF Chen, S Ramalingam and ML Chye. 2008. Overexpression of membrane-associated acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsis. Plant Journal 54: 141-151.
- Y Guan, S Ramalingam, D Nagegowda, P Taylor and ML Chye. 2008. Brassica juncea chitinase BjCHI1 inhibits growth of fungal phytopathogens and agglutinates Gram-negative bacteria. J Exp Bot 59: 3475-3484.
- QF Chen, S Xiao and ML Chye. 2008. Overexpression of the Arabidopsis 10-kilodalton acyl-CoA-binding protein ACBP6 enhances freezing tolerance. Plant Physiology 148: 304-315.
- S Xiao and ML Chye. 2008. Arabidopsis ACBP1 overexpressors are Pb(II)-tolerant and accumulate Pb(II). Plant Signaling & Behavior 3: 693-695.
- QF Chen, S Xiao and ML Chye. 2008. Arabidopsis ACBP6 is an acyl-CoA-binding protein associated with phospholipid metabolism. Plant Signaling & Behavior 3: 1019-1020.
- S Xiao, HY Li, JP Zhang, SW Chan and ML Chye. 2008b. Arabidopsis acyl-CoA-binding proteins ACBP4 and ACBP5 are localized to the cytosol and ACBP4 depletion affects membrane lipid composition. Plant Mol Biol 68: 571-583.
- HY Li, S Xiao and ML Chye. 2008. Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein. J Exp Bot 59: 3997-4006.
- Y Guan and ML Chye.2008. A Brassica juncea chitinase with two-chitin binding domains shows anti-microbial properties against phytopathogens and Gram-negative bacteria. Plant Signaling & Behavior 3: 1103-1105.
- W Ubhayasekara, CM Tang, SWT Ho, G Berlund, T Bergfors, ML Chye and SL Mowbray. 2007. Crystal structures of a family 19 chitinase from Brassica juncea show flexibility of binding cleft loops. FEBS J 274: 3695-3703.
- R Welti, J Shah, W Li, M Li, J Chen, JJ Burke, ML Fauconnier, K Chapman, ML Chye and X Wang. 2007. Plant lipidomics: discerning biological function by profiling plant complex lipids using mass spectrometry. Frontiers in Bioscience 12: 2494-2506.
- W Ubhayasekara, CM Tang, R Rawat, SWT Ho, ML Chye and SL Mowbray. 2007. Involvement of loops in catalysis in family 19 chitinases. Advances in Chitin Science 10: 147-152.
- KC Leung, HY Li, S Xiao, MH Tse, ML Chye. 2006. Arabidopsis ACBP3 is an extracellularly targeted acyl-CoA-binding protein. Planta 223: 871-881.
- SF Sin, EC Yeung, ML Chye. 2006. Down-regulation of Solanum americanum genes encoding proteinase inhibitor II causes defective seed development. Plant Journal 46: 58-70.
- Y Zhou, MYT Lee, JMH Ng, ML Chye, WK Yip, SY Zee and E Lam. 2006. A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is antigenic in mice. World Journal of Gastroenterology 12: 306-312.
- MYT Lee, Y Zhou, AH Chu, RWM Lung, ML Chye, WK Yip, SY Zee and E Lam. 2006. Expression of viral capsid protein antigen against Epstein-Barr virus in plastids of Nicotiana tabacum cv. SR1. Biotech Bioeng 94: 1129-1137.
- HY Li, S Ramalingam and ML Chye. 2006. Accumulation of recombinant SARS-CoV spike protein in plant cytosol and chloroplasts indicate potential development of plant-derived vaccines. Expt Biol Medicine 231: 1346-1352.
- F Pojer, JL Ferrer, SB Richard, DA Nagegowda, ML Chye, TJ Bach and JP Noel. 2006. Structural basis for the design of potent and species specific inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A synthases. Proc Natl Acad Sci USA 103: 11491-11496.
- ML Chye, KJ Zhao, ZM He, S Ramalingam and KL Fung. 2005. An agglutinating chitinase BjCHI1 with two chitin-binding domains confers fungal protection in transgenic potato. Planta 220: 717-730.
- D Nagegowda, S Ramalingam, A Hemmerlin, TJ Bach and ML Chye. 2005. Brassica juncea HMG-CoA synthase: localization of mRNA and protein. Planta 221: 844-856.
- R Rawat, ZF Xu, KM Yao and ML Chye. 2005. Identification of cis-elements in ethylene and circadian regulation of gene encoding Solanum melongena cysteine proteinase. Plant Mol Biol 57: 629-643.
- ZF Xu, WL Teng and ML Chye. 2004. Inhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a. Planta 218: 623-629.
- HY Li and ML Chye. 2004. Arabidopsis acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element binding protein AtEBP via its ankyrin repeats. Plant Mol Biol 54: 233-243.
- KC Leung, HY Li, G Mishra and ML Chye. 2004. ACBP4 and ACBP5, novel Arabidopsis acyl-CoA-binding proteins with kelch motifs that bind oleoyl-CoA. Plant Mol Biol 55: 297-309.
- SF Sin and ML Chye. 2004. Expression of proteinase inhibitor II proteins during floral development in Solanum americanum flowers. Planta 219: 1010-1022.
- D Nagegowda, TJ Bach and ML Chye. 2004. Brassica juncea HMG-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes. Biochem J 383: 517-527.
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