School of Biological Sciences
The University of Hong Kong
Professor M. L. Chye
Wilson and Amelia Wong Professor in Plant Biotechnology
Tel. +852 2299-0319
M.L. Chye, the Wilson and Amelia Wong Professor in Plant Biotechnology at HKU, completed her PhD on a Commonwealth Scholarship at the University of Melbourne and received postdoctoral training in Plant Molecular Biology at the Rockefeller University (New York) and the Institute of Molecular and Cell Biology (Singapore). She joined the University of Hong Kong in 1993 was promoted to Professor in 2005.
She has been awarded an Edward Clarence Dyason Universitas 21 Fellowship (2004/05), an Outstanding University Researcher Award (2006/07), a Croucher Senior Research Fellowship (2007/08), and an Eileen Mary Harris Scholarship (2013). She serves on the editorial boards of Plant Molecular Biology (Springer), Planta (Springer), Frontiers in Plant Metabolism & Chemodiversity, Frontiers in Plant Cell Biology, and Frontiers in Plant Physiology, was Chair of the 4th Asian Symposium on Plant Lipids (2011) and Chair of the 12th International Symposium on Biocatalysis and Agricultural Biotechnology (2016).
Laboratory of Plant Molecular Biology
The main focus of the Chye Lab is to understand the function and mechanism of action of stress-induced plant proteins, particularly plant acyl-CoA-binding proteins (ACBPs). We intend to use them to generate transformed plants that can better tolerate abiotic and biotic stresses since these stresses account for ~40 % loss in crop productivity. Ultimately, investigations on plant ACBPs, and others, will be applied to agriculture and phytoremediation.
Our projects have been supported by the Wilson and Amelia Wong Endowment Fund, the Research Grants Council of Hong Kong, the “Centre for Organelle Biogenesis and Function” Area of Excellence AoE/M-05/12 (http://www.cuhk.edu.hk/centre/iCell/index.html), the Plant and Agricultural Biotechnology Area of Excellence (http://www.cuhk.edu.hk/bio/aoe/) and the State Key Lab of Agrobiotechnology, Chinese University of Hong Kong (http://www.cuhk.edu.hk/ipmbab/SKL/index.html). We intend to extend our findings on the model plant, Arabidopsis thaliana, to rice which is an important staple crop in Asia.
We collaborate with the Saunders, Wang, and Lo Labs within the “Plant Evolution and Adaptation” Strategic Research Area (http://www.biosch.hku.hk/staff/mlc/mlc.html), and plant biologists at the Chinese University of Hong Kong on projects funded by the Research Grants Council including the “Centre for Organelle Biogenesis and Function” Area of Excellence AoE/M-05/12, “Centre for Genomic Studies on Plant-Environment Interaction for Sustainable Agriculture and Food Security” Area of Excellence AoE/M-403/16”, Collaborative Group Research CUHK2/CRF/11G, and General Research Fund 468013M. We also work with the “Food” eSRT (http://www.rss.hku.hk/strategic-research/community/food) and the “Earth as a Habitable Planet” eSRT (http://www.rss.hku.hk/strategic-research/environment/earth-as-a-habitable-planet) at HKU.
Arabidopsis acyl-CoA-binding proteins (ACBPs) and stress tolerance
We are studying a family of plant ACBPs which bind acyl-CoA esters and transport them within the plant cell. In Arabidopsis and rice, six genes encode four structurally distinct classes of ACBPs (Leung et al., 2004; Xiao and Chye, 2009; 2011b; Meng et al., 2010; 2014). In Arabidopsis the six members are
In our attempt to understand the function of Arabidopsis ACBPs, we have identified the amino acid residues in the acyl-CoA-binding domain that are important in binding acyl-CoA esters (Chye et al., 2000; Leung et al., 2004; 2006). Some ACBPs can also bind phospholipids (Chen et al., 2008; Du et al., 2010; Chen et al., 2010; Xiao et al., 2010). On lipid analysis, Arabidopsis acbp mutants and transgenic Arabidopsis lines overexpressing ACBPs showed alterations in lipid composition (Xiao et al., 2008b; 2010; Chen et al., 2008; 2010; Du et al., 2010, 2013a, 2013b). ACBPs with ankyrin repeats (Li and Chye, 2004; Gao et al., 2009; 2010; Du et al., 2013b) and kelch motifs (Leung et al., 2004; Li et al., 2008; Xiao et al., 2008b) have been demonstrated to mediate protein-protein interactions.
We have observed that certain ACBPs are induced by various forms of abiotic and biotic stresses (Xiao et al., 2008a; Li et al., 2008; Chen et al., 2008; Gao et al., 2009; 2010; Xiao and Chye; 2010, 2011a; Zheng et al., 2012; Du et al., 2013a, 2013b). Subsequently, when these ACBPs were overexpressed in transgenic plants, the resultant lines were conferred stress tolerance. ACBP6-overexpressors were freezing tolerant (Chen et al., 2008; Liao et al., 2014; US Patent No. 8378172) and ACBP2-overexpressors were drought tolerant (Du et al., 2013b; US Patent Application 13,667,569). ACBP1- and ACBP2-overexpressors also displayed tolerance to heavy metal and oxidative stresses (Xiao et al., 2008; Gao et al., 2009; 2010; Du et al., 2014). Interestingly, ACBP1-overexpressors accumulate Pb(II) in shoots making ACBP1 applicable for phytoremediation, a low-cost solar-driven process that removes pollutants from the environment in situ (Xiao et al., 2008a, Xiao and Chye, 2008; US Patent No. 7,880,053).
Stress-inducible HMGS, an enzyme in plant isoprenoid metabolism
We are investigating the role of 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) in plant isoprenoid metabolism. HMGS is an enzyme in the cytosolic mevalonate pathway which produces sterols, sesquiterpenes and polyterpenes. Its expression is stress-inducible and is highest during early development in flower, seed and seedling (Alex et al., 1999). Four isogenes encoding HMGS are differentially expressed in Brassica juncea (Nagegowda et al., 2005).
Using green fluorescent protein fusions, B. juncea HMGS1 (BjHMGS) has been subcellularly localized to the cytosol (Nagegowda et al., 2005). We have biochemically purified and characterized His-tagged recombinant BjHMGS expressed in Escherichia coli, presenting a first detailed characterization of a plant HMGS, and the amino acids involved in catalysis were identified by site-directed mutagenesis (Nagegowda et al., 2004). In collaboration with the Bach Lab and the Noel Lab, the crystal structure of Brassica HMGS covalently bound to specific inhibitor F-244 has been determined, providing a first approach towards the design of more potent cholesterol-lowering drugs and antibiotics that target the mevalonate pathway (Pojer et al., 2006).
When mutant and wild-type BjHMGS were expressed in transgenic Arabidopsis, the genes of the sterol biosynthetic pathway were upregulated resulting in an overaccumulation of phytosterols (stimasterols, campesterol and stigmasterols), and the HMGS-overexpressing lines were better protected against oxidative stress and Botrytis infection (Wang et al., 2012). Furthermore, the overexpression of HMGS variant S359A in a model plant from the family Solanaceae, not only resulted in phytosterol accumulation but showed enhanced plant growth, pod size and seed yield (Liao et al., 2014; US Patent Application 14/260,561). The potential of S359A in boosting seed yield may now be tested in food crops.
Expression of siRNAs and heterologous proteins in genetically-transformed plants
Some of the strategies currently used to engineer crops to stress tolerance exploit the natural mechanisms used by the plant. We have isolated defense-related genes from tropical plants for expression in transgenic crops. To this end, we have cloned and characterized cDNAs encoding beta-1, 3-glucanase from Hevea brasiliensis (Chye and Cheung, 1995, Plant Mol Biol 29: 347-402) and an unusual Brassica juncea chitinase with two chitin-binding domains, designated BjCHI1 (Zhao and Chye, 1999; Fung et al., 2002). Potato transgenic for both proteins was protected from Rhizoctonia solani invasion (Chye et al., 2005). Besides displaying anti-fungal properties, BjCHI1 with its two chitin-binding domains can agglutinate Gram-negative bacteria (Tang et al., 2004; Guan et al., 2008). BjCHI1-susceptible fungal and bacterial phytopathogens have been identified for future applications in agriculture (Guan et al., 2008; Guan and Chye, 2008; US Patent No. 6,956,147). To better understand the function of plant chitinases in catalysis, we have examined their crystal structures with the Mowbray Lab (Ubhayasekara et al., 2007; 2009).
Research on proteinase inhibitor protein SaPIN2a from a weed, Solanum americanum (Xu et al., 2001), has not only led to the production of transgenic lettuce that are insect-resistant but that also show inhibition of endogenous trypsin- and chymotrysin-like activities (Xu et al., 2004). Thus, SaPIN2a can be used to protect heterologously expressed proteins by minimizing protein degradation and optimizing protein yield in transgenic plant bioreactors (US Patent No. 7,256,327). SaPIN2a and SaPIN2b function endogenously by inhibiting proteinase activities in phloem and floral development (Xu et al., 2001; Sin and Chye, 2004). We have used RNAi-based gene silencing to show that SaPIN2a and SaPIN2b are essential for seed development (Sin et al., 2006). A reduction in seed set was observed in PIN2-RNAi transgenic S. americanum lines; aborted seeds in transgenic fruits had an abnormal endothelium, suggesting that the endothelium allows proper endosperm and embryo formation through its ability to produce a proteinase inhibitor (Sin et al., 2006).
In other projects related to plant biotechnology, we have explored the use of transgenic plants as bioreactors by the characterization of promoters that direct expression in seeds (Chen et al., 2013), and the production of vaccines using nuclear transformation and plastid transformation (Zhou et al., 2006; Lee et al., 2006; Li et al., 2006; Li and Chye, 2009; Chye et al., Chinese Patent No. zl200480039355.6).
- SC Lung, P Liao, E Yeung, AS Hsiao, Y Xue, and ML Chye. 2017. Arabidopsis acyl-CoA-binding protein ACBP1 modulates sterol synthesis during embryogenesis. Plant Physiology 174: 1420-1435, DOI:10.1104/pp.17.00412
- ZH Guo, WH. Chan, GK. Kong, Q Hao and ML Chye. 2017. The first plant acyl-CoA-binding protein structures: the close homologues OsACBP1 and OsACBP2 from rice. Acta Cryst. D73: 438-448, DOI:10.1107/S2059798317004193
- ZW Ye, J Xu, J Shi, D Zhang and ML Chye. 2017. Kelch-motif containing acyl-CoA binding proteins AtACBP4 and AtACBP5 are differentially expressed and function in floral lipid metabolism. Plant Mol. Biol. 93: 209-225, DOI: 10.1007/s11103-016-0557-5
- ZY Du, T Arias, W Meng and ML Chye. 2016. Plant acyl-CoA-binding proteins: an emerging family in plant development and stress responses. Prog. Lipid Res 63: 165-181, DOI: 10.1016/j.plipres.2016.06.002
- ZW Ye, SC Lung, TH Hu, QF Chen, YL Suen, M Wang, S Hoffmann-Benning, E Yeung and ML Chye. 2016. Arabidopsis acyl-CoA-binding protein ACBP6 localizes in the phloem and affects jasmonate composition. Plant Mol. Biol. 92: 717–730, DOI: 10.1007/s11103-016-0541-0.
- Y Xue, SC Lung and ML Chye. 2016. Present status and future prospects of transgenic approaches for drought tolerance. In “Drought Tolerance in Plants, Vol 2: Molecular and Genetic Perspectives" (ISBN 978-3-319-32421-0) edited by LS Tran, DJ Burritt, MA Hossain, SH Wani and S Bhattacharjee. Springer, USA, Chapter 20, p. 549-569.
- P Liao, A Hemmerlin, TJ Bach and ML Chye. 2016. The potential of the mevalonate pathway for enhanced isoprenoid production. Biotechnology Advances 34: 697-713.
- SC Lung and ML Chye. 2016. Acyl-CoA-binding proteins in plant development. In “Lipids in plant and algae development” (ISBN 9783319259796) edited by Y Nakamura and Y Li-Beisson. Springer. Chapter 15, pp. 363-404. http://www.springer.com/us/book/9783319259772
- JA Aznar-Moreno, M Venegas-Caleron, ZY Du, R Garces, JA Tanner, ML Chye, E Martinez-Force and JJ Salas. 2016. Characterization of a small acyl-CoA-binding protein (ACBP) from Helianthus annuus L. and its binding affinities. Plant Physiology and Biochemistry 102: 141-150.
- SC Lung and ML Chye. 2016. Deciphering the roles of acyl-CoA-binding proteins in plant cells. Protoplasma 253: 1177-119, DOI 10.1007/s00709-015-0882-6.
- SC Lung and ML Chye. 2016. The binding versatility of plant acyl-CoA-binding proteins and their significance in lipid metabolism. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1861: 409-1421, Special Issue on Plant Lipid Biology 10.1016/j.bbalip.2015.12.018
- ZW Ye and ML Chye. 2016. Plant cytosolic acyl-CoA-binding proteins. Lipids 51: 1-13, 50th Commemorative Issue DOI 10.1007/s11745-015-4103-z
- ZY Du, MX Chen, QF Chen, JD Gu and ML Chye. 2015. Expression of Arabidopsis acyl-CoA-binding proteins AtACBP1 and AtACBP4 confers Pb(II) accumulation in Brassica juncea roots. Plant Cell Environ. 38: 101–117.
- AS Hsiao, RP Haslam, LV Michaelson, P Liao, QF Chen, S Sooriyaarachchi, SL Mowbray, JA Napier, JA Tanner and ML Chye. 2015. Arabidopsis cytosolic acyl-CoA-binding proteins ACBP4, ACBP5 and ACBP6 have overlapping but distinct roles in seed development. Bioscience Reports 34: 865–877.
- AS Hsiao, EC Yeung, ZW Ye and ML Chye. 2015. The Arabidopsis cytosolic acyl-CoA-binding proteins play combinatory roles in pollen development. Plant Cell Physiol. 56: 322-333.
- YL Yung, MY Cheung, R Miao, YH Fong, KP Li, MH Yu, ML Chye, KB Wong and HM Lam. 2015. Site-directed mutagenesis shows the significance of interactions with phospholipids and the G-protein OsYchF1 on the physiological functions of the rice GTPase-Activating Protein 1 (OsGAP1). J. Biol. Chem. 290: 23984-23996.
- YT Lai, YY Chang, L Hu, Y Yang, ZY Du, H Li, JA Tanner, ML Chye, C Qian and H Sun. 2015. Rapid labeling of intracellular His-tagged proteins in living cells. Proc. Natl Acad. Sci. USA 112: 2948-2953.
- W Meng, AS Hsiao, C Gao, L Jiang and ML Chye. 2014. The subcellular localization of rice ACBPs indicates that OsACBP6-GFP is targeted to the peroxisome. New Phytologist 203: 469-482.
- W Meng and ML Chye. 2014. Rice acyl-CoA-binding proteins OsACBP4 and OsACBP5 are differentially localized in the endoplasmic reticulum of transgenic Arabidopsis. Plant Signaling & Behavior 9:e29544.
- P Liao, QF Chen and ML Chye. 2014. Transgenic Arabidopsis flowers overexpressing acyl-CoA-binding protein ACBP6 are freezing tolerant. Plant Cell Physiol. 55: 1055-1071.
- P Liao, H Wang, M Wang, AS Hsiao, TJ Bach, ML Chye. 2014. Transgenic tobacco overexpressing Brassica juncea HMG-CoA synthase 1 shows increased plant growth, pod size and seed yield. PLOS ONE 9: e98264 (http://dx.plos.org/10.1371/journal.pone.0098264).
- P Liao, H Wang, A Hemmerlin, DA Nagegowda, TJ Bach, M Wang and ML Chye. 2014. Past achievements, current status and future perspectives of studies on 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) in the mevalonate (MVA) pathway. Plant Cell Reports 33: 1005-1022.
- MX Chen, SX Zheng, YN Yang,C Xu, Y Wang, JS Liu, WD Yang, ML Chye and HY Li. 2014. Strong seed-specific protein expression from the Vigna radiata storage protein 8SGα promoter in transgenic Arabidopsis seeds. Journal of Biotechnology 174: 49-56.
- MX Chen, SC Lung, ZY Du and ML Chye. 2014. Engineering plants to tolerate abiotic stresses. In a special issue on "Transformation methods and innovation platforms in plant biotechnology". Biocatalysis and Agricultural Biotechnology 3: 81-87, edited by I. Kalvochuk and R. Weselake. Elsevier.
- Y Xue, S Xiao, J Kim, SC Lung, L Chen, JA Tanner, MC Suh and ML Chye. 2014. Arabidopsis membrane-associated acyl-CoA-binding protein AtACBP1 is involved in stem cuticle formation. J. Exp. Bot. 18: 5473-5483.
- AS Hsiao, RP Haslam. LV Michaelson. P Liao, JA Napier and ML Chye. 2014. Gene expression in plant lipid metabolism in Arabidopsis seedlings. PLOS ONE 9:e107372 (http://dx.plos.org/10.1371/journal.pone.0107372)
- ZY Du, MX Chen, QF Chen, S Xiao and ML Chye. 2013a. Arabidopsis Acyl-CoA-Binding Protein ACBP1 participates in the regulation of seed germination and seedling development. Plant Journal 74: 294-309.
- ZY Du, MX Chen, QF Chen, S Xiao and ML Chye. 2013b. Overexpression of Arabidopsis Acyl-CoA-Binding Protein ACBP2 enhances drought tolerance. Plant Cell Environ. 36: 300-314.
- KW Kwan, ZW Ye, ML Chye and AHW Ngan. 2013. A mathematical model on water redistribution mechanism of the seismonastic movement of Mimosa pudica. Biophysical J. 105: 266-275
- MX Chen, YN Yang, SX Zheng, C Xu, Y Wang, JS Liu, WD Yang, ML Chye and HY Li. 2013. A Vigna radiata 8S globulin α’ promoter drives efficient expression of GUS in Arabidopsis cotyledonary embryos. J. Agri. Food Chem. 61: 6423–6429.
- ZY Du and ML Chye. 2013. Interactions between Arabidopsis acyl-CoA-binding proteins and protein partners. Planta 238: 239-245.
- F Zhu, L Li, PY Lam, MX Chen, ML Chye and C Lo. 2013. Sorghum extracellular leucine-rich repeat protein SbLRR2 mediates lead tolerance in transgenic Arabidopsis. Plant Cell Physiol. 54: 1549-1559.
- SX Zheng, S Xiao and ML Chye. 2012. The gene encoding Arabidopsis Acyl-CoA-Binding Protein 3 is pathogen-inducible and subject to circadian regulation. J. Exp. Bot. 63: 2985-3000.
- H Wang, DA Nagegowda, R Rawat, P Bouvier-Navé, D Guo, TJ Bach and ML Chye. 2012. Overexpression of Brassica juncea wild-type and mutant HMG-CoA synthase 1 in Arabidopsis up-regulates genes in sterol biosynthesis and enhances sterol production and stress tolerance. Plant Biotechnol. J. 10: 31-42.
- S Xiao and ML Chye. 2011a. Overexpression of Arabidopsis acyl-CoA-binding protein 3 enhances NPR1-dependent plant resistance to Pseudomonas syringae pv. tomato DC3000 Plant Physiology 156: 2069-2081.
- S Xiao and ML Chye. 2011b. New roles for acyl-CoA-binding proteins in plant development, stress responses and lipid metabolism Prog. Lipid Res. 50: 141-151.
- W Meng, YCF Su, RMK Saunders and ML Chye. 2011. The rice ACBP gene family: phylogeny, expression and functional analysis. New Phytologist 189: 1170-1184.
- S Xiao, W Gao, QF Chen, SW Chan, SX Zheng, J Ma, M Wang, R Welti and ML Chye. 2010. Overexpression of Arabidopsis acyl-CoA-binding protein ACBP3 promotes starvation-induced and age-dependent leaf senescence. Plant Cell 22: 1463-1482.
- W Gao, HY Li, S Xiao and ML Chye. 2010. Protein interactors of acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis. Plant Signaling & Behavior 5: 1025-1027.
- S Xiao and ML Chye. 2010. The Arabidopsis thaliana ACBP3 regulates leaf senescence by modulating phospholipid metabolism and ATG8 stability. Autophagy 6: 802-804
- W Gao, HY Li, S Xiao and ML Chye. 2010. Acyl-CoA-binding protein 2 binds lysophospholipase 2 and lysoPC to promote tolerance to cadmium-induced oxidative stress in transgenic Arabidopsis. Plant Journal 62: 989-1003.
- ZY Du, S Xiao, QF Chen and ML Chye. 2010. Arabidopsis acyl-CoA-binding proteins ACBP1 and ACBP2 show different roles in freezing stress. Plant Signaling & Behavior 5: 607-609.
- QF Chen, S Xiao, W Qi, G Mishra, J Ma, M Wang and ML Chye.2010. The Arabidopsis acbp1acbp2 double mutant lacking acyl-CoA-binding proteins ACBP1 and ACBP2 is embryo lethal. New Phytologist 186: 843-855.
"As simple as ACB – new insights into the role of acyl-CoA-binding proteins in Arabidopsis" JA Napier and RP Haslam. New Phytologist 186: 781-783.
- ZY Du, S Xiao, QF Chen and ML Chye.2010. Depletion of the membrane-associated acyl-CoA-binding protein ACBP1 confers freezing tolerance in Arabidopsis. Plant Physiology 152: 1585-1597.
- W Gao, S Xiao, HY Li, SW Tsao and ML Chye. 2009. Arabidopsis thaliana acyl-CoA-binding protein ACBP2 interacts with a heavy-metal-binding farnesylated protein AtFP6. New Phytologist 181: 89-102.
- HY Li and ML Chye. 2009. Use of green fluorescent protein to investigate expression of plant-derived vaccines. In “Viral applications of green fluorescent protein”, Methods in Molecular Biology 515: Chapter 19, pp. 275-287, edited by BA Hicks.
- S Xiao and ML Chye. 2009. An Arabidopsis family of six acyl-CoA-binding proteins has three cytosolic members. Plant Physiol Biochem 47: 479-484.
- S Xiao, QF Chen and ML Chye. 2009. Light-regulated Arabidopsis ACBP4 and ACBP5 encode cytosolic acyl-CoA-binding proteins that bind phosphatidylcholine and oleoyl-CoA ester. Plant Physiol Biochem 47: 926-933.
- S Xiao, QF Chen and ML Chye. 2009. Expression of ACBP4 and ACBP5 proteins is modulated by light in Arabidopsis. Plant Signaling & Behavior 4: 1063-1065.
- W Ubhayasekera, R Rawat, SWT Ho, M Wiweger, S Von Arnold, ML Chye and SL Mowbray. 2009. The first crystal structures of a family 19 class IV chitinase: the enzyme from Norway spruce. Plant Mol Biol 71: 277-289.
- S Xiao, W Gao, QF Chen, S Ramalingam and ML Chye. 2008. Overexpression of membrane-associated acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsis. Plant Journal 54: 141-151.
- Y Guan, S Ramalingam, D Nagegowda, P Taylor and ML Chye. 2008. Brassica juncea chitinase BjCHI1 inhibits growth of fungal phytopathogens and agglutinates Gram-negative bacteria. J Exp Bot 59: 3475-3484.
- QF Chen, S Xiao and ML Chye. 2008. Overexpression of the Arabidopsis 10-kilodalton acyl-CoA-binding protein ACBP6 enhances freezing tolerance. Plant Physiology 148: 304-315.
- S Xiao and ML Chye. 2008. Arabidopsis ACBP1 overexpressors are Pb(II)-tolerant and accumulate Pb(II). Plant Signaling & Behavior 3: 693-695.
- QF Chen, S Xiao and ML Chye. 2008. Arabidopsis ACBP6 is an acyl-CoA-binding protein associated with phospholipid metabolism. Plant Signaling & Behavior 3: 1019-1020.
- S Xiao, HY Li, JP Zhang, SW Chan and ML Chye. 2008b. Arabidopsis acyl-CoA-binding proteins ACBP4 and ACBP5 are localized to the cytosol and ACBP4 depletion affects membrane lipid composition. Plant Mol Biol 68: 571-583.
- HY Li, S Xiao and ML Chye. 2008. Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein. J Exp Bot 59: 3997-4006.
- Y Guan and ML Chye.2008. A Brassica juncea chitinase with two-chitin binding domains shows anti-microbial properties against phytopathogens and Gram-negative bacteria. Plant Signaling & Behavior 3: 1103-1105.
- W Ubhayasekara, CM Tang, SWT Ho, G Berlund, T Bergfors, ML Chye and SL Mowbray. 2007. Crystal structures of a family 19 chitinase from Brassica juncea show flexibility of binding cleft loops. FEBS J 274: 3695-3703.
- R Welti, J Shah, W Li, M Li, J Chen, JJ Burke, ML Fauconnier, K Chapman, ML Chye and X Wang. 2007. Plant lipidomics: discerning biological function by profiling plant complex lipids using mass spectrometry. Frontiers in Bioscience 12: 2494-2506.
- W Ubhayasekara, CM Tang, R Rawat, SWT Ho, ML Chye and SL Mowbray. 2007. Involvement of loops in catalysis in family 19 chitinases. Advances in Chitin Science 10: 147-152.
- KC Leung, HY Li, S Xiao, MH Tse, ML Chye. 2006. Arabidopsis ACBP3 is an extracellularly targeted acyl-CoA-binding protein. Planta 223: 871-881.
- SF Sin, EC Yeung, ML Chye. 2006. Down-regulation of Solanum americanum genes encoding proteinase inhibitor II causes defective seed development. Plant Journal 46: 58-70.
- Y Zhou, MYT Lee, JMH Ng, ML Chye, WK Yip, SY Zee and E Lam. 2006. A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is antigenic in mice. World Journal of Gastroenterology 12: 306-312.
- MYT Lee, Y Zhou, AH Chu, RWM Lung, ML Chye, WK Yip, SY Zee and E Lam. 2006. Expression of viral capsid protein antigen against Epstein-Barr virus in plastids of Nicotiana tabacum cv. SR1. Biotech Bioeng 94: 1129-1137.
- HY Li, S Ramalingam and ML Chye. 2006. Accumulation of recombinant SARS-CoV spike protein in plant cytosol and chloroplasts indicate potential development of plant-derived vaccines. Expt Biol Medicine 231: 1346-1352.
- F Pojer, JL Ferrer, SB Richard, DA Nagegowda, ML Chye, TJ Bach and JP Noel. 2006. Structural basis for the design of potent and species specific inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A synthases. Proc Natl Acad Sci USA 103: 11491-11496.
- ML Chye, KJ Zhao, ZM He, S Ramalingam and KL Fung. 2005. An agglutinating chitinase BjCHI1 with two chitin-binding domains confers fungal protection in transgenic potato. Planta 220: 717-730.
- D Nagegowda, S Ramalingam, A Hemmerlin, TJ Bach and ML Chye. 2005. Brassica juncea HMG-CoA synthase: localization of mRNA and protein. Planta 221: 844-856.
- R Rawat, ZF Xu, KM Yao and ML Chye. 2005. Identification of cis-elements in ethylene and circadian regulation of gene encoding Solanum melongena cysteine proteinase. Plant Mol Biol 57: 629-643.
- ZF Xu, WL Teng and ML Chye. 2004. Inhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a. Planta 218: 623-629.
- HY Li and ML Chye. 2004. Arabidopsis acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element binding protein AtEBP via its ankyrin repeats. Plant Mol Biol 54: 233-243.
- KC Leung, HY Li, G Mishra and ML Chye. 2004. ACBP4 and ACBP5, novel Arabidopsis acyl-CoA-binding proteins with kelch motifs that bind oleoyl-CoA. Plant Mol Biol 55: 297-309.
- SF Sin and ML Chye. 2004. Expression of proteinase inhibitor II proteins during floral development in Solanum americanum flowers. Planta 219: 1010-1022.
- D Nagegowda, TJ Bach and ML Chye. 2004. Brassica juncea HMG-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes. Biochem J 383: 517-527.
- CM Tang, ML Chye, S Ramalingam, SW Ouyang, KJ Zhao, W Ubhayasekera and S Mowbray. 2004. Functional analysis of the chitin-binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1. Plant Mol Biol 56: 285-298.
- ZF Xu, ML Chye, HY Li, FX Xu and KM Yao. 2003. G-box binding coincides with increased S. melongena cysteine proteinase expression in senescent fruits and circadian-regulated leaves. Plant Mol Biol 51: 9-19.
- HY Li and ML Chye. 2003. Membrane localization of Arabidopsis acyl-CoA-binding protein ACBP2. Plant Mol Biol 51: 483-492.
- KL Fung, KJ Zhao, ZM He and ML Chye. 2002. Tobacco-expressed Brassica juncea chitinase BjCHI1 shows antifungal activity in vitro. Plant Mol Biol 50: 283-294.
- ZF Xu, WQ Qi, XZ Ouyang, E Yeung and ML Chye. 2001. A proteinase inhibitor II of Solanum americanum is expressed in phloem. Plant Mol Biol 47: 727-738.
- D Alex, TJ Bach and ML Chye. 2000. Expression of Brassica juncea 3-hydroxy-3-methylglutaryl-CoA synthase is developmentally regulated and stress-responsive. Plant Journal 22: 415-426.
- ML Chye, HY Li and MH Yung. 2000. Single amino acids substitutions at the acyl-CoA-binding domain interrupt 14[C]palmitoyl-CoA binding of ACBP2, an Arabidopsis acyl-CoA-binding protein with ankyrin repeats. Plant Mol Biol: 44: 711-721.
- KJ Zhao and ML Chye. 1999. Methyl jasmonate induces expression of a Brassica juncea chitinase with two chitin-binding domains. Plant Mol Biol 40: 1009-1018.
- ML Chye, BQ Huang and SY Zee. 1999. Isolation of a gene encoding Arabidopsis membrane-associated acyl-CoA binding protein and immunolocalization of its gene product. Plant Journal 18: 205-214.
- FX Xu and ML Chye. 1999. Expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal. Plant Journal 17: 321-327.
Last modified: 3/7/2013
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