Lee Tsz On, Leo

 B.Sc.(Hon), M.Phil., Ph.D. (Hong Kong)

email: ltolee2@hkucc.hku.hk

Research interests:

  1. Transcriptional regulation of secretin and secretin receptor

  2. Molecular evolution of peptides from the secretin/GIP/GHRH superfamily

  3. The involvement of RASSF1A in Nasopharyngeal carcinoma

  4. The pleiotropic functions of secretin


Publications:

  1. JYS Chu, LTO Lee, CH Lai, H Vaudry, YS Chan, WH Yung, BKC Chow 2009 Secretin as a neurohypophysial factor regulating body fluid homeostasis. Proc. Natl. Acad. Sci. U.S.A. 106(37):15961-6.

  2. VHY Lee, BKC Chow, KW Lo, LS Chow, C Man, SW Tsao, LTO Lee # 2009 Regulation of RASSF1A in nasopharyngeal cells and its response to UV irradiation. Gene 443(1-2):55-63

  3. IPY Lam*, LTO Lee*, Choi HS, Alpini G, Chow BKC 2009 Bile Acids Inhibit Duodenal Secretin Expression via Orphan Nuclear Receptor Small Heterodimer Partner (SHP). Am J Physiol Gastrointest Liver Physiol. 297(1):G90-97

  4. VHY Lee, LTO Lee, BKC Chow 2008 Gonadotropin-releasing hormone: regulation of the GnRH gene. FEBS J. 275(22):5458-5478

  5. LTO Lee, JKV Tam, DW Chan, BKC Chow 2009 Molecular cloning and mRNA distribution of pituitary adenylate cyclase-activating polypeptide (PACAP)/ PACAP-related peptide in the lungfish. Ann. N. Y. Acad. Sci.  1163: 204-214

  6. LTO Lee, IPY Lam, BKC Chow 2008 A Functional Variable Number of Tandem Repeats is Located at the 5’ Flanking Region of the Human Secretin Gene Plays a Down Regulatory Role in Expression. J Mol Neurosci. 36(1-3):125-31

  7. FKY Siu*, LTO Lee*, BKC Chow 2008 Southwestern Blotting in Investigating Transcriptional Regulation. Nat Protoc. 3(1): 51-58

  8. LTO Lee, FKY Siu, JKV Tam, ITY Lau, H Vaudry, MCM Lin, AOL Wong, BKC Chow 2007 Discovery of growth hormone-releasing hormones and receptors in non-mammalian vertebrates. Proc. Natl. Acad. Sci. U.S.A. 104(7):2133-8.

  9. JKV Tam, LTO Lee, BKC Chow 2007 PACAP-related peptide (PRP) – Molecular evolution and potential functions. Peptides 28(9): 1920-29

  10. RLC Hoo, KYY Chan, FKY Leung, LTO Lee, PCK Leung, BKC Chow 2007 Involvement of NF-kappa B Subunit p65 and Retinoic Acid Receptors RAR alpha and RXR alpha in the Transcriptional Regulation of the Human GnRH II Gene. FEBS J. 274(11):2695-706.

  11. C Man, J Rosa, LTO Lee, VHY Lee, BKC Chow, KW Lo, S Doxsey, ZG Wu, YL Kwong, DY Jin, ALM Cheung, SW Tsao 2007 Latent membrane protein 1 suppresses RASSF1A expression, disrupts microtubule structures and induces chromosomal aberrations in human epithelial cells. Oncogene. 26(21):3069-80.

  12. JY Chu, LTO Lee, FK Siu, BKC Chow 2006 The secretin/pituitary adenylate cyclase-activating polypeptide/vasoactive intestinal polypeptide superfamily in the central nervous system. Curr. Med. Chem. - Central Nervous System Agents 6: 27-57(31).

  13. LTO Lee, Tan-Un KC, BKC Chow 2006 Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1 Ann. N. Y. Acad. Sci. 1070: 393-398.

  14. LTO Lee, VH Lee, PY Yuan, BKC Chow 2006 Identification of repressor element 1 in secretin/PACAP/VIP genes Ann. N. Y. Acad. Sci. 1070: 388-392.

  15. IPY Lam, LTO Lee, HS Choi, BKC Chow 2006 Localization of small heterodimer partner (SHP) with the secretin in mouse duodenal cells Ann. N. Y. Acad. Sci. 1070: 371-375.

  16. LTO Lee, KC Tan-Un, MCM Lin, BKC Chow 2005 Retinoic acid activates human secretin gene expression by Sp-proteins and Nuclear Factor I in neuronal SH-SY5Y cells.  Journal of Neurochemistry 93:339-350.

  17. LTO Lee, TU Tan, RTK Pang, DTW Lam, BKC Chow 2004 Regulation of the human secretin gene is controlled by the combined effects of CpG methylation, Sp1/Sp3 ratio and the E-Box element. Molecular Endocrinology 18(7):1740-1755.

  18. RTK Pang, LTO Lee, SSM Ng, WH Yung, BKC Chow 2004 CpG methylation and transcription factors Sp1 and Sp3 regulate the expression of the human secretin receptor gene. Molecular Endocrinology 18(2): 471-483.

  19. LTO Lee, G Nong, YH Chan, DL Tse, CH Cheng 2001 Molecular cloning of a teleost growth hormone receptor and its functional interaction with human growth hormone. Gene 270:121-129.

  20. SS Ng, BKC Chow, RT Pang, LTO Lee, KY Chan, WH Yung 2001 Expression and cellular distribution of secretin and its receptor in the cerebellum. Perspective in Comparative Endocrinology: Unity Diversity 621-626.

  21. DL Tse, BKC Chow, CB Chan, LTO Lee, CH Cheng 2000 Molecular cloning and expression studies of a prolactin receptor in goldfish (Carassius auratus). Life Sci. 66:593-605.

* Co-first author publication
# Corresponding author publication

　

RESEARCH GRANTS RECORD

As Principal Investigator:

GRF:
  1. Implications of PACAP/VIP receptor gene duplications in early vertebrates
    HKD$ 850,230 GRF 769609
  2. A role of secretin in the subfornical organ in sensing osmotic changes
    HKD$ 1,372,474 GRF 768608
CRCG:
  1. The role of glucagon receptor family in the unique hyperglycermic activity of teleosts glucagon-like peptide 1
    HKD 93,000 CRCG 11159073
    (Seed funding programme for basic research)
  2. Interactions of secretin and orexin in controlling food intake
    HKD$ 100,000 CRCG 11159084 
    (Seed funding programme for basic research)
  3. Molecular evolution of PACAP and VIP receptors in agnathan: Searching for the ancestral PACAP/VIP receptor gene of vertebrates.
    HKD$ 240,000 CRCG 10208128 
    (Seed funding programme for basic rbbyayaesearch)
  4. Transcriptional regulation of a nasopharyngeal carcinoma tumor suppressor: RASSF1A 
    HKD$ 35,000 CRCG 10205784.
    (Small project funding)
As Co-Investigator:
  1. Neuron-restrictive silencer factor (NRSF) regulates cell-specific expression of the human secretin receptor gene
    HKD$100,000 CRCG 11159075